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Journal of Virology, July 2005, p. 8620-8624, Vol. 79, No. 13
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.13.8620-8624.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

A Small Interfering RNA Targeting Coxsackievirus B3 Protects Permissive HeLa Cells from Viral Challenge

Jeonghyun Ahn,1 Eun Seok Jun,2,3 Hui Sun Lee,1 Seung Yong Yoon,4 DongHou Kim,4,5 Chul-Hyun Joo,1,5 Yoo Kyum Kim,1,5 and Heuiran Lee1,5*

Departments of Microbiology,1 Anatomy & Cell Biology,4 Research Institute for Biomacromolecules, University of Ulsan College of Medicine,5 Department of Medicine, Sungkyunkwan University School of Medicine,2 Cardiac and Vascular Center, Samsung Medical Center, Seoul, Korea3

Received 10 December 2004/ Accepted 1 March 2005

We examined the ability of small interfering RNAs (siRNAs) to disrupt infection by coxsackievirus B3 (CVB3). The incorporation of siRNAs dramatically decreased cell death in permissive HeLa cells in parallel with a reduction in viral replication. Three of four siRNAs had potent anti-CVB3 activity. The present study thus demonstrates that the antiviral effect is due to the downregulation of viral replication. In addition, an effective CVB3-specific siRNA had similar antiviral effects in other related enteroviruses possessing sequence homology in the targeted region. Because the CVB3-specific siRNA is effective against other enteroviruses, siRNAs have potential for a universal antienterovirus strategy.

* Corresponding author. Mailing address: University of Ulsan College of Medicine, Department of Microbiology, University of Ulsan College of Medicine, Songpa, P.O. Box 145, Seoul, Korea. Phone: 82-2-3010-4286. Fax: 82-2-485-2320. E-mail: heuiran{at}amc.seoul.kr.

Journal of Virology, July 2005, p. 8620-8624, Vol. 79, No. 13
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.13.8620-8624.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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