This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Loh, J.
Right arrow Articles by Virgin, H. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Loh, J.
Right arrow Articles by Virgin, H. W., IV

 Previous Article

Journal of Virology, January 2005, p. 661-667, Vol. 79, No. 1
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.1.661-667.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Natural Killer Cells Utilize both Perforin and Gamma Interferon To Regulate Murine Cytomegalovirus Infection in the Spleen and Liver

Joy Loh,1 Dortha T. Chu,2 Andrew K. O'Guin,1 Wayne M. Yokoyama,2 and Herbert W. Virgin IV1*

Departments of Pathology and Immunology and Molecular Microbiology,1 Howard Hughes Medical Institute, Division of Rheumatology, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri2

Received 9 March 2004/ Accepted 26 August 2004

Natural killer (NK) cells are critical for innate regulation of the acute phase of murine cytomegalovirus (MCMV) infection and have been reported to utilize perforin (Pfp)- and gamma interferon (IFN-{gamma})-dependent effector mechanisms in an organ-specific manner to regulate MCMV infection in the spleen and liver. In this study, we further examined the roles of NK cells, Pfp, and IFN-{gamma} in innate immunity to MCMV infection. With the recently described NK cell-deficient (NKD) mouse, we confirmed previous findings that NK cells, but not NKT cells, are required for control of the acute phase of MCMV infection in spleen and liver cells. Interestingly, we found that Pfp and IFN-{gamma} are each important for regulating MCMV replication in both the spleen and the liver. Moreover, NK cells can regulate MCMV infection in the spleens and livers of Pfp–/– mice in a Pfp-independent manner and can use an IFN-{gamma}-independent mechanism to control MCMV infection in IFN-{gamma}–/– mice. Thus, contrary to previous reports, NK cells utilize both Pfp and IFN-{gamma} to control MCMV infection in the spleen and liver.

* Corresponding author. Mailing address: Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Ave., Box 8118, St. Louis, MO 63110. Phone: (314) 362-9223. Fax: (314) 362-4096. E-mail: virgin{at}immunology.wustl.edu.

Journal of Virology, January 2005, p. 661-667, Vol. 79, No. 1
0022-538X/05/$08.00+0     doi:10.1128/JVI.79.1.661-667.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lee, S.-H., Kim, K.-S., Fodil-Cornu, N., Vidal, S. M., Biron, C. A. (2009). Activating receptors promote NK cell expansion for maintenance, IL-10 production, and CD8 T cell regulation during viral infection. JEM 206: 2235-2251 [Abstract] [Full Text]  
  • Orr, M. T., Sun, J. C., Hesslein, D. G.T., Arase, H., Phillips, J. H., Takai, T., Lanier, L. L. (2009). Ly49H signaling through DAP10 is essential for optimal natural killer cell responses to mouse cytomegalovirus infection. JEM 206: 807-817 [Abstract] [Full Text]  
  • Thirion, G., Coutelier, J.-P. (2009). Production of protective gamma interferon by natural killer cells during early mouse hepatitis virus infection. J. Gen. Virol. 90: 442-447 [Abstract] [Full Text]  
  • Fodil-Cornu, N., Lee, S.-H., Belanger, S., Makrigiannis, A. P., Biron, C. A., Buller, R. M., Vidal, S. M. (2008). Ly49h-Deficient C57BL/6 Mice: A New Mouse Cytomegalovirus-Susceptible Model Remains Resistant to Unrelated Pathogens Controlled by the NK Gene Complex. J. Immunol. 181: 6394-6405 [Abstract] [Full Text]  
  • Davis, A. H., Guseva, N. V., Ball, B. L., Heusel, J. W. (2008). Characterization of Murine Cytomegalovirus m157 from Infected Cells and Identification of Critical Residues Mediating Recognition by the NK Cell Receptor Ly49H. J. Immunol. 181: 265-275 [Abstract] [Full Text]  
  • Strowig, T., Brilot, F., Munz, C. (2008). Noncytotoxic Functions of NK Cells: Direct Pathogen Restriction and Assistance to Adaptive Immunity. J. Immunol. 180: 7785-7791 [Abstract] [Full Text]  
  • Andrews, D. M., Andoniou, C. E., Fleming, P., Smyth, M. J., Degli-Esposti, M. A. (2008). The Early Kinetics of Cytomegalovirus-Specific CD8+ T-Cell Responses Are Not Affected by Antigen Load or the Absence of Perforin or Gamma Interferon. J. Virol. 82: 4931-4937 [Abstract] [Full Text]  
  • Bekiaris, V., Timoshenko, O., Hou, T. Z., Toellner, K., Shakib, S., Gaspal, F., McConnell, F. M., Parnell, S. M., Withers, D., Buckley, C. D., Sweet, C., Yokoyama, W. M., Anderson, G., Lane, P. J. L. (2008). Ly49H+ NK Cells Migrate to and Protect Splenic White Pulp Stroma from Murine Cytomegalovirus Infection. J. Immunol. 180: 6768-6776 [Abstract] [Full Text]  
  • Guia, S., Cognet, C., de Beaucoudrey, L., Tessmer, M. S., Jouanguy, E., Berger, C., Filipe-Santos, O., Feinberg, J., Camcioglu, Y., Levy, J., Al Jumaah, S., Al-Hajjar, S., Stephan, J.-L., Fieschi, C., Abel, L., Brossay, L., Casanova, J.-L., Vivier, E. (2008). A role for interleukin-12/23 in the maturation of human natural killer and CD56+ T cells in vivo. Blood 111: 5008-5016 [Abstract] [Full Text]  
  • Rosenberger, C. M., Clark, A. E., Treuting, P. M., Johnson, C. D., Aderem, A. (2008). ATF3 regulates MCMV infection in mice by modulating IFN-{gamma} expression in natural killer cells. Proc. Natl. Acad. Sci. USA 105: 2544-2549 [Abstract] [Full Text]  
  • Iizuka, K., Nakajima, C., Iizuka, Y.-M., Takase, M., Kato, T., Noda, S., Tanaka, K., Kanagawa, O. (2007). Protection from Lethal Infection by Adoptive Transfer of CD8 T Cells Genetically Engineered to Express Virus-Specific Innate Immune Receptor. J. Immunol. 179: 1122-1128 [Abstract] [Full Text]  
  • Crozat, K., Hoebe, K., Ugolini, S., Hong, N. A., Janssen, E., Rutschmann, S., Mudd, S., Sovath, S., Vivier, E., Beutler, B. (2007). Jinx, an MCMV susceptibility phenotype caused by disruption of Unc13d: a mouse model of type 3 familial hemophagocytic lymphohistiocytosis. JEM 204: 853-863 [Abstract] [Full Text]  
  • van den Pol, A. N., Robek, M. D., Ghosh, P. K., Ozduman, K., Bandi, P., Whim, M. D., Wollmann, G. (2007). Cytomegalovirus InducesInterferon-Stimulated Gene Expression and Is Attenuated by Interferon in the Developing Brain. J. Virol. 81: 332-348 [Abstract] [Full Text]  
  • Ilyinskii, P. O., Wang, R., Balk, S. P., Exley, M. A. (2006). CD1d Mediates T-Cell-Dependent Resistance to Secondary Infection with Encephalomyocarditis Virus (EMCV) In Vitro and Immune Response to EMCV Infection In Vivo. J. Virol. 80: 7146-7158 [Abstract] [Full Text]  
  • Szomolanyi-Tsuda, E., Liang, X., Welsh, R. M., Kurt-Jones, E. A., Finberg, R. W. (2006). Role for TLR2 in NK Cell-Mediated Control of Murine Cytomegalovirus In Vivo. J. Virol. 80: 4286-4291 [Abstract] [Full Text]  
  • Iversen, A.-C., Norris, P. S., Ware, C. F., Benedict, C. A. (2005). Human NK Cells Inhibit Cytomegalovirus Replication through a Noncytolytic Mechanism Involving Lymphotoxin-Dependent Induction of IFN-{beta}. J. Immunol. 175: 7568-7574 [Abstract] [Full Text]  
  • Welstead, G. G., Iorio, C., Draker, R., Bayani, J., Squire, J., Vongpunsawad, S., Cattaneo, R., Richardson, C. D. (2005). Measles virus replication in lymphatic cells and organs of CD150 (SLAM) transgenic mice. Proc. Natl. Acad. Sci. USA 102: 16415-16420 [Abstract] [Full Text]  
  • Pao, L. I., Sumaria, N., Kelly, J. M., Dommelen, S. v., Cretney, E., Wallace, M. E., Anthony, D. A., Uldrich, A. P., Godfrey, D. I., Papadimitriou, J. M., Mullbacher, A., Degli-Esposti, M. A., Smyth, M. J. (2005). Functional Analysis of Granzyme M and Its Role in Immunity to Infection. J. Immunol. 175: 3235-3243 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»