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Journal of Virology, March 2004, p. 3196-3199, Vol. 78, No. 6
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.6.3196-3199.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Complete Protection from Papillomavirus Challenge after a Single Vaccination with a Vesicular Stomatitis Virus Vector Expressing High Levels of L1 Protein

Anjeanette Roberts,1 Jon D. Reuter,2 Jean H. Wilson,2 Stuart Baldwin,1 and John K. Rose1*

Department of Pathology,1 Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut 065102

Received 28 August 2003/ Accepted 3 November 2003

We generated an attenuated, recombinant vesicular stomatitis virus (VSV) expressing high levels of the cottontail rabbit papillomavirus (CRPV) L1 protein from an upstream site in the VSV genome. Rabbits vaccinated once with this VSV-L1 recombinant produced high levels of anti-L1 antibody and were completely protected against papilloma formation after challenge with CRPV. In contrast, animals vaccinated only once with a VSV vector expressing lower levels of L1 from a downstream site in the VSV genome generated lower levels of L1 antibody and demonstrated only incomplete protection from papilloma formation after challenge. We conclude that the level of L1 protein expression is critical in generating complete immunity with a single-dose vaccine.

* Corresponding author. Mailing address: Department of Pathology, BML 342, 310 Cedar St., New Haven, CT 06510. Phone: (203) 785-6184. Fax: (203) 785-7467. E-mail: john.rose{at}yale.edu.

Journal of Virology, March 2004, p. 3196-3199, Vol. 78, No. 6
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.6.3196-3199.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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