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Journal of Virology, March 2004, p. 3145-3148, Vol. 78, No. 6
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.6.3145-3148.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Amino-Terminal Region of Major Capsid Protein P3 Is Essential for Self-Assembly of Single-Shelled Core-Like Particles of Rice Dwarf Virus

Kyoji Hagiwara,1 Takahiko Higashi,1 Naoyuki Miyazaki,2 Hisashi Naitow,3 R. Holland Cheng,4 Atsushi Nakagawa,2 Hiroshi Mizuno,5 Tomitake Tsukihara,2 and Toshihiro Omura1*

Laboratory of Virology, National Agricultural Research Center, Tsukuba, Ibaraki 305-8666,1 Institute for Protein Research, Osaka University, Suita, Osaka 565-0871,2 Division of Bio-Crystallography Technology, RIKEN Harima Institute, Sayo-gun, Hyogo 679-5148,3 National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan,5 Department of Biosciences at Novum, Karolinska Institute, 14157 Huddinge, Sweden4

Received 26 August 2003/ Accepted 21 November 2003

The core protein P3 of Rice dwarf virus constructs asymmetric dimers, one of which is inserted by the amino-terminal region of another P3 protein. The P3 proteins with serial amino-terminal deletions, expressed in a baculovirus system, formed particles with gradually decreasing stability. The capacity for self-assembly disappeared when 52 of the amino-terminal amino acids had been deleted. These results demonstrated that insertion of the amino-terminal arm of one P3 protein into another appears to play an important role in stabilizing the core particles.

* Corresponding author. Mailing address: National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, Japan. Phone: 81-298-38-8932. Fax: 81-298-38-8929. E-mail: toomura{at}affrc.go.jp.

Journal of Virology, March 2004, p. 3145-3148, Vol. 78, No. 6
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.6.3145-3148.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Miyazaki, N., Uehara-Ichiki, T., Xing, L., Bergman, L., Higashiura, A., Nakagawa, A., Omura, T., Cheng, R. H. (2008). Structural Evolution of Reoviridae Revealed by Oryzavirus in Acquiring the Second Capsid Shell. J. Virol. 82: 11344-11353 [Abstract] [Full Text]  


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