This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takada, A.
Right arrow Articles by Kawaoka, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takada, A.
Right arrow Articles by Kawaoka, Y.

 Previous Article  |  Next Article 

Journal of Virology, March 2004, p. 2943-2947, Vol. 78, No. 6
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.6.2943-2947.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Human Macrophage C-Type Lectin Specific for Galactose and N-Acetylgalactosamine Promotes Filovirus Entry

Ayato Takada,1,2 Kouki Fujioka,3 Makoto Tsuiji,3 Akiko Morikawa,3 Nobuaki Higashi,3 Hideki Ebihara,4 Darwyn Kobasa,4 Heinz Feldmann,5 Tatsuro Irimura,3 and Yoshihiro Kawaoka1,2,4*

Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo 108-8639,1 CREST, Japan Science and Technology Corporation, Saitama 332-0012,2 Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033, Japan,3 Department of Pathobiological Sciences, University of Wisconsin, Madison, Wisconsin 53706,4 Special Pathogens Program, National Microbiology Laboratory, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba R3E 3R2, Canada5

Received 10 July 2003/ Accepted 24 November 2003

Filoviruses cause lethal hemorrhagic disease in humans and nonhuman primates. An initial target of filovirus infection is the mononuclear phagocytic cell. Calcium-dependent (C-type) lectins such as dendritic cell- or liver/lymph node-specific ICAM-3 grabbing nonintegrin (DC-SIGN or L-SIGN, respectively), as well as the hepatic asialoglycoprotein receptor, bind to Ebola or Marburg virus glycoprotein (GP) and enhance the infectivity of these viruses in vitro. Here, we demonstrate that a recently identified human macrophage galactose- and N-acetylgalactosamine-specific C-type lectin (hMGL), whose ligand specificity differs from DC-SIGN and L-SIGN, also enhances the infectivity of filoviruses. This enhancement was substantially weaker for the Reston and Marburg viruses than for the highly pathogenic Zaire virus. We also show that the heavily glycosylated, mucin-like domain on the filovirus GP is required for efficient interaction with this lectin. Furthermore, hMGL, like DC-SIGN and L-SIGN, is present on cells known to be major targets of filoviruses (i.e., macrophages and dendritic cells), suggesting a role for these C-type lectins in viral replication in vivo. We propose that filoviruses use different C-type lectins to gain cellular entry, depending on the cell type, and promote efficient viral replication.

* Corresponding author. Mailing address: Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. Phone: 608-265-4925. Fax: 608-265-5622. E-mail: kawaoka{at}ims.u-tokyo.ac.jp.

Journal of Virology, March 2004, p. 2943-2947, Vol. 78, No. 6
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.6.2943-2947.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wertheim, J. O., Worobey, M. (2009). Relaxed Selection and the Evolution of RNA Virus Mucin-Like Pathogenicity Factors. J. Virol. 83: 4690-4694 [Abstract] [Full Text]  
  • Dube, D., Brecher, M. B., Delos, S. E., Rose, S. C., Park, E. W., Schornberg, K. L., Kuhn, J. H., White, J. M. (2009). The Primed Ebolavirus Glycoprotein (19-Kilodalton GP1,2): Sequence and Residues Critical for Host Cell Binding. J. Virol. 83: 2883-2891 [Abstract] [Full Text]  
  • Saba, K., Denda-Nagai, K., Irimura, T. (2009). A C-Type Lectin MGL1/CD301a Plays an Anti-Inflammatory Role in Murine Experimental Colitis. Am. J. Pathol. 174: 144-152 [Abstract] [Full Text]  
  • Sakakura, M., Oo-Puthinan, S., Moriyama, C., Kimura, T., Moriya, J., Irimura, T., Shimada, I. (2008). Carbohydrate Binding Mechanism of the Macrophage Galactose-type C-type Lectin 1 Revealed by Saturation Transfer Experiments. J. Biol. Chem. 283: 33665-33673 [Abstract] [Full Text]  
  • Kaletsky, R. L., Simmons, G., Bates, P. (2007). Proteolysis of the Ebola Virus Glycoproteins Enhances Virus Binding and Infectivity. J. Virol. 81: 13378-13384 [Abstract] [Full Text]  
  • Brindley, M. A., Hughes, L., Ruiz, A., McCray, P. B. Jr., Sanchez, A., Sanders, D. A., Maury, W. (2007). Ebola Virus Glycoprotein 1: Identification of Residues Important for Binding and Postbinding Events. J. Virol. 81: 7702-7709 [Abstract] [Full Text]  
  • Dowling, W., Thompson, E., Badger, C., Mellquist, J. L., Garrison, A. R., Smith, J. M., Paragas, J., Hogan, R. J., Schmaljohn, C. (2007). Influences of Glycosylation on Antigenicity, Immunogenicity, and Protective Efficacy of Ebola Virus GP DNA Vaccines. J. Virol. 81: 1821-1837 [Abstract] [Full Text]  
  • Sano, Y., Usami, K., Izawa, R., Denda-Nagai, K., Higashi, N., Kimura, T., Suzuki, N., Irimura, T. (2007). Properties of Blocking and Non-blocking Monoclonal Antibodies Specific for Human Macrophage Galactose-type C-type Lectin (MGL/ClecSF10A/CD301). J Biochem 141: 127-136 [Abstract] [Full Text]  
  • Shimojima, M., Takada, A., Ebihara, H., Neumann, G., Fujioka, K., Irimura, T., Jones, S., Feldmann, H., Kawaoka, Y. (2006). Tyro3 family-mediated cell entry of ebola and marburg viruses.. J. Virol. 80: 10109-10116 [Abstract] [Full Text]  
  • Marzi, A., Akhavan, A., Simmons, G., Gramberg, T., Hofmann, H., Bates, P., Lingappa, V. R., Pohlmann, S. (2006). The Signal Peptide of the Ebolavirus Glycoprotein Influences Interaction with the Cellular Lectins DC-SIGN and DC-SIGNR.. J. Virol. 80: 6305-6317 [Abstract] [Full Text]  
  • Kuhn, J. H., Radoshitzky, S. R., Guth, A. C., Warfield, K. L., Li, W., Vincent, M. J., Towner, J. S., Nichol, S. T., Bavari, S., Choe, H., Aman, M. J., Farzan, M. (2006). Conserved Receptor-binding Domains of Lake Victoria Marburgvirus and Zaire Ebolavirus Bind a Common Receptor. J. Biol. Chem. 281: 15951-15958 [Abstract] [Full Text]  
  • Sanchez, A. J., Vincent, M. J., Erickson, B. R., Nichol, S. T. (2006). Crimean-Congo Hemorrhagic Fever Virus Glycoprotein Precursor Is Cleaved by Furin-Like and SKI-1 Proteases To Generate a Novel 38-Kilodalton Glycoprotein. J. Virol. 80: 514-525 [Abstract] [Full Text]  
  • Ji, X., Olinger, G. G., Aris, S., Chen, Y., Gewurz, H., Spear, G. T. (2005). Mannose-binding lectin binds to Ebola and Marburg envelope glycoproteins, resulting in blocking of virus interaction with DC-SIGN and complement-mediated virus neutralization. J. Gen. Virol. 86: 2535-2542 [Abstract] [Full Text]  
  • Kwan, W.-H., Helt, A.-M., Maranon, C., Barbaroux, J.-B., Hosmalin, A., Harris, E., Fridman, W. H., Mueller, C. G. F. (2005). Dendritic Cell Precursors Are Permissive to Dengue Virus and Human Immunodeficiency Virus Infection. J. Virol. 79: 7291-7299 [Abstract] [Full Text]  
  • van Vliet, S. J., van Liempt, E., Saeland, E., Aarnoudse, C. A., Appelmelk, B., Irimura, T., Geijtenbeek, T. B. H., Blixt, O., Alvarez, R., van Die, I., van Kooyk, Y. (2005). Carbohydrate profiling reveals a distinctive role for the C-type lectin MGL in the recognition of helminth parasites and tumor antigens by dendritic cells. Int Immunol 17: 661-669 [Abstract] [Full Text]  
  • Manicassamy, B., Wang, J., Jiang, H., Rong, L. (2005). Comprehensive Analysis of Ebola Virus GP1 in Viral Entry. J. Virol. 79: 4793-4805 [Abstract] [Full Text]  
  • Marzi, A., Gramberg, T., Simmons, G., Moller, P., Rennekamp, A. J., Krumbiegel, M., Geier, M., Eisemann, J., Turza, N., Saunier, B., Steinkasserer, A., Becker, S., Bates, P., Hofmann, H., Pohlmann, S. (2004). DC-SIGN and DC-SIGNR Interact with the Glycoprotein of Marburg Virus and the S Protein of Severe Acute Respiratory Syndrome Coronavirus. J. Virol. 78: 12090-12095 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»