This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Iwamoto, T.
Right arrow Articles by Nakai, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Iwamoto, T.
Right arrow Articles by Nakai, T.

 Previous Article  |  Next Article 

Journal of Virology, February 2004, p. 1256-1262, Vol. 78, No. 3
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.3.1256-1262.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification of Host-Specificity Determinants in Betanodaviruses by Using Reassortants between Striped Jack Nervous Necrosis Virus and Sevenband Grouper Nervous Necrosis Virus

Tokinori Iwamoto,1,{dagger} Yasushi Okinaka,2* Kazuyuki Mise,3 Koh-Ichiro Mori,1 Misao Arimoto,1 Tetsuro Okuno,3 and Toshihiro Nakai2

Kamiura Station, Japan Sea-Farming Association, Oita 879-2602,1 Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528,2 Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan3

Received 27 June 2003/ Accepted 4 October 2003

Betanodaviruses, the causal agents of viral nervous necrosis in marine fish, have bipartite positive-sense RNAs as genomes. The larger genomic segment, RNA1 (3.1 kb), encodes an RNA-dependent RNA polymerase, and the smaller genomic segment, RNA2 (1.4 kb), codes for the coat protein. Betanodaviruses have marked host specificity, although the primary structures of the viral RNAs and encoded proteins are similar among betanodaviruses. However, no mechanism underlying the host specificity has yet been reported. To evaluate viral factors that control host specificity, we first constructed a cDNA-mediated infectious RNA transcription system for sevenband grouper nervous necrosis virus (SGNNV) in addition to that for striped jack nervous necrosis virus (SJNNV), which was previously established by us. We then tested two reassortants between SJNNV and SGNNV for infectivity in the host fish from which they originated. When striped jack and sevenband grouper larvae were bath challenged with the reassortant virus comprising SJNNV RNA1 and SGNNV RNA2, sevenband groupers were killed exclusively, similar to inoculation with SGNNV. Conversely, inoculations with the reassortant virus comprising SGNNV RNA1 and SJNNV RNA2 killed striped jacks but did not affect sevenband groupers. Immunofluorescence microscopic studies using anti-SJNNV polyclonal antibodies revealed that both of the reassortants multiplied in the brains, spinal cords, and retinas of infected fish, similar to infections with parental virus inoculations. These results indicate that viral RNA2 and/or encoded coat protein controls host specificity in SJNNV and SGNNV.

* Corresponding author. Mailing address: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan. Phone: 81-824-24-7978. Fax: 81-824-22-7059. E-mail: okinaka{at}hiroshima-u.ac.jp.

{dagger} Present address: University of Alabama at Birmingham, Birmingham, AL 35294.

Journal of Virology, February 2004, p. 1256-1262, Vol. 78, No. 3
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.3.1256-1262.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Olveira, J. G., Souto, S., Dopazo, C. P., Thiery, R., Barja, J. L., Bandin, I. (2009). Comparative analysis of both genomic segments of betanodaviruses isolated from epizootic outbreaks in farmed fish species provides evidence for genetic reassortment. J. Gen. Virol. 90: 2940-2951 [Abstract] [Full Text]  
  • Gomez, D. K., Baeck, G. W., Kim, J. H., Choresca, C. H. Jr., Park, S. C. (2008). Molecular detection of betanodavirus in wild marine fish populations in Korea. jvdi 20: 38-44 [Abstract] [Full Text]  
  • Furusawa, R., Okinaka, Y., Nakai, T. (2006). Betanodavirus infection in the freshwater model fish medaka (Oryzias latipes).. J. Gen. Virol. 87: 2333-2339 [Abstract] [Full Text]  
  • Iwamoto, T., Mise, K., Takeda, A., Okinaka, Y., Mori, K.-I., Arimoto, M., Okuno, T., Nakai, T. (2005). Characterization of Striped jack nervous necrosis virus subgenomic RNA3 and biological activities of its encoded protein B2. J. Gen. Virol. 86: 2807-2816 [Abstract] [Full Text]  
  • Liu, W., Hsu, C.-H., Hong, Y.-R., Wu, S.-C., Wang, C.-H., Wu, Y.-M., Chao, C.-B., Lin, C.-S. (2005). Early endocytosis pathways in SSN-1 cells infected by dragon grouper nervous necrosis virus. J. Gen. Virol. 86: 2553-2561 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»