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Journal of Virology, December 2004, p. 13366-13369, Vol. 78, No. 23
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.23.13366-13369.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection and Analysis of Six Lizard Adenoviruses by Consensus Primer PCR Provides Further Evidence of a Reptilian Origin for the Atadenoviruses

James F. X. Wellehan,1* April J. Johnson,1 Balázs Harrach,2 Mária Benkö,2 Allan P. Pessier,3 Calvin M. Johnson,4,{dagger} Michael M. Garner,5 April Childress,1 and Elliott R. Jacobson1

Department of Small Animal Clinical Sciences,1 Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, Florida,4 Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary,2 Department of Pathology, Center for Reproduction of Endangered Species, Zoological Society of San Diego, San Diego, California,3 Northwest ZooPath, Monroe, Washington5

Received 29 May 2004/ Accepted 9 July 2004

A consensus nested-PCR method was designed for investigation of the DNA polymerase gene of adenoviruses. Gene fragments were amplified and sequenced from six novel adenoviruses from seven lizard species, including four species from which adenoviruses had not previously been reported. Host species included Gila monster, leopard gecko, fat-tail gecko, blue-tongued skink, Tokay gecko, bearded dragon, and mountain chameleon. This is the first sequence information from lizard adenoviruses. Phylogenetic analysis indicated that these viruses belong to the genus Atadenovirus, supporting the reptilian origin of atadenoviruses. This PCR method may be useful for obtaining templates for initial sequencing of novel adenoviruses.

* Corresponding author. Mailing address: Zoological Medicine Service, Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610. Phone: (352) 392-4700. Fax: (352) 392-4877. E-mail: wellehanj{at}mail.vetmed.ufl.edu.

{dagger} Present address: Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849.

Journal of Virology, December 2004, p. 13366-13369, Vol. 78, No. 23
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.23.13366-13369.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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