This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishikawa, H.
Right arrow Articles by Kobayashi, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishikawa, H.
Right arrow Articles by Kobayashi, M.

 Previous Article  |  Next Article 

Journal of Virology, November 2004, p. 12703-12708, Vol. 78, No. 22
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.22.12703-12708.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Host Range Factor 1 from Lymantria dispar Nucleopolyhedrovirus (NPV) Is an Essential Viral Factor Required for Productive Infection of NPVs in IPLB-Ld652Y Cells Derived from L. dispar

Hiroki Ishikawa,1 Motoko Ikeda,2 Cristiano A. Felipe Alves,1,{dagger} Suzanne M. Thiem,3 and Michihiro Kobayashi1*

Laboratory of Biodynamics,1 Laboratory of Sericulture and Entomoresources, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Japan,2 Department of Entomology and Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan3

Received 10 May 2004/ Accepted 29 June 2004

Host range factor 1 (HRF-1) of Lymantria dispar multinucleocapsid nucleopolyhedrovirus promotes Autographa californica MNPV replication in nonpermissive Ld652Y cells derived from L. dispar. Here we demonstrate that restricted Hyphantria cunea NPV replication in Ld652Y cells was not due to apoptosis but was likely due to global protein synthesis arrest that could be restored by HRF-1. Our data also showed that HRF-1 promoted the production of progeny virions for two other baculoviruses, Bombyx mori NPV and Spodoptera exigua MNPV, whose replication in Ld652Y cells is limited to replication of viral DNA without successful production of infectious progeny virions. Thus, HRF-1 is an essential viral factor required for productive infection of NPVs in Ld652Y cells.

* Corresponding author. Mailing address: Laboratory of Biodynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan. Phone and Fax: 81-52-789-4038. E-mail: michihir{at}agr.nagoya-u.ac.jp.

{dagger} Present address: Department of Microbiology, University of Otago, Dunedin, New Zealand.

Journal of Virology, November 2004, p. 12703-12708, Vol. 78, No. 22
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.22.12703-12708.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ikeda, M., Shikata, M., Shirata, N., Chaeychomsri, S., Kobayashi, M. (2006). Gene organization and complete sequence of the Hyphantria cunea nucleopolyhedrovirus genome. J. Gen. Virol. 87: 2549-2562 [Abstract] [Full Text]  
  • Wilson, J. A., Forney, S. D., Ricci, A. M., Allen, E. G., Hefferon, K. L., Miller, L. K. (2005). Expression and Mutational Analysis of Autographa californica Nucleopolyhedrovirus HCF-1: Functional Requirements for Cysteine Residues. J. Virol. 79: 13900-13914 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»