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Journal of Virology, November 2004, p. 12665-12667, Vol. 78, No. 22
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.22.12665-12667.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Neuraminidase Is Important for the Initiation of Influenza Virus Infection in Human Airway Epithelium

Mikhail N. Matrosovich,1,2* Tatyana Y. Matrosovich,1 Thomas Gray,3 Noel A. Roberts,4 and Hans-Dieter Klenk1

Institute of Virology, Philipps University, Marburg, Germany,1 M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides, Moscow, Russia,2 Laboratory of Molecular Carcinogenesis, National Institutes of Environmental Health Sciences, Research Triangle Park, North Carolina,3 Roche Products, Ltd., Welwyn Garden City, Hertsfordshire, United Kingdom4

Received 28 April 2004/ Accepted 7 July 2004

Influenza virus neuraminidase (NA) plays an essential role in release and spread of progeny virions, following the intracellular viral replication cycle. To test whether NA could also facilitate virus entry into cell, we infected cultures of human airway epithelium with human and avian influenza viruses in the presence of the NA inhibitor oseltamivir carboxylate. Twenty- to 500-fold less cells became infected in drug-treated versus nontreated cultures (P < 0.0001) 7 h after virus application, indicating that the drug suppressed the initiation of infection. These data demonstrate that viral NA plays a role early in infection, and they provide further rationale for the prophylactic use of NA inhibitors.

* Corresponding author. Mailing address: Institute of Virology, Philipps University, Robert Koch Str. 17, 35037 Marburg, Germany. Phone: 49-(6421)-286-5166. Fax: 49-(6421)-286-8962. E-mail: Mikhail.Matrosovich{at}med.uni-marburg.de.

Journal of Virology, November 2004, p. 12665-12667, Vol. 78, No. 22
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.22.12665-12667.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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