This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gianni, T.
Right arrow Articles by Menotti, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gianni, T.
Right arrow Articles by Menotti, L.

 Previous Article  |  Next Article 

Journal of Virology, November 2004, p. 12268-12276, Vol. 78, No. 22
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.22.12268-12276.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Entry of Herpes Simplex Virus Mediated by Chimeric Forms of Nectin1 Retargeted to Endosomes or to Lipid Rafts Occurs through Acidic Endosomes

Tatiana Gianni, Gabriella Campadelli-Fiume, and Laura Menotti*

Section on Microbiology and Virology, Department of Experimental Pathology, University of Bologna, Bologna, Italy

Received 27 April 2004/ Accepted 17 June 2004

Herpes simplex virus (HSV) enters cells by fusion with target membranes, commonly the plasma membrane. In some cells, including CHO cells expressing the nectin1 or herpesvirus entry mediator receptors, entry occurs through an endocytic route. We report the following results. (i) When expressed in J cells, nectin1 and HVEM mediated a pathway of entry insensitive to endosome acidification inhibitors. (ii) A chimeric nectin1 receptor competent for endosomal uptake by fusion of the nectin1 ectodomain with the transmembrane sequence and cytoplasmic tail of the epidermal growth factor receptor (EGFR1) (nectin1-EGFR1) and chimeric nectin1 sorted to lipid rafts by a glycosylphosphatidylinositol anchor mediated endocytic entry blocked by the early endosome inhibitor wortmannin and by the endosome acidification inhibitors bafilomycin and NH4Cl. (iii) Entry mediated by nectin1-EGFR1 was selectively inhibited by AG1478, a tyrosine phosphorylation inhibitor that targets the EGFR1 cytoplasmic tail and blocks the signaling pathway that culminates in clathrin-dependent uptake of the receptor into endosomes. We draw the following conclusions. (i) The same receptor may initiate different routes of infection, depending on the cell in which it is expressed. Hence, the cell is a determinant that controls whether a given receptor initiates a plasma membrane or an endocytic route of entry. (ii) Receptors whose physiology involves uptake into endosomes or sorting to lipid rafts are suitable to serve as HSV receptors. (iii) Structural features of the receptors are additional determinants that control whether HSV entry occurs at the plasma membrane or at endosomes. These findings are relevant to studies of HSV retargeting to specific receptors.

* Corresponding author. Mailing address: Section on Microbiology and Virology, Department of Experimental Pathology, University of Bologna, Via San Giacomo 12, 40126 Bologna, Italy. Phone: 39 051 2094731. Fax: 39 051 2094735. E-mail: menotti{at}alma.unibo.it.

Journal of Virology, November 2004, p. 12268-12276, Vol. 78, No. 22
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.22.12268-12276.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Petermann, P., Haase, I., Knebel-Morsdorf, D. (2009). Impact of Rac1 and Cdc42 Signaling during Early Herpes Simplex Virus Type 1 Infection of Keratinocytes. J. Virol. 83: 9759-9772 [Abstract] [Full Text]  
  • Hahn, A., Birkmann, A., Wies, E., Dorer, D., Mahr, K., Sturzl, M., Titgemeyer, F., Neipel, F. (2009). Kaposi's Sarcoma-Associated Herpesvirus gH/gL: Glycoprotein Export and Interaction with Cellular Receptors. J. Virol. 83: 396-407 [Abstract] [Full Text]  
  • Van de Walle, G. R., Peters, S. T., VanderVen, B. C., O'Callaghan, D. J., Osterrieder, N. (2008). Equine Herpesvirus 1 Entry via Endocytosis Is Facilitated by {alpha}V Integrins and an RSD Motif in Glycoprotein D. J. Virol. 82: 11859-11868 [Abstract] [Full Text]  
  • Akkarawongsa, R., Potocky, T. B., English, E. P., Gellman, S. H., Brandt, C. R. (2008). Inhibition of Herpes Simplex Virus Type 1 Infection by Cationic {beta}-Peptides. Antimicrob. Agents Chemother. 52: 2120-2129 [Abstract] [Full Text]  
  • Delboy, M. G., Roller, D. G., Nicola, A. V. (2008). Cellular Proteasome Activity Facilitates Herpes Simplex Virus Entry at a Postpenetration Step. J. Virol. 82: 3381-3390 [Abstract] [Full Text]  
  • Nagel, C.-H., Dohner, K., Fathollahy, M., Strive, T., Borst, E. M., Messerle, M., Sodeik, B. (2008). Nuclear Egress and Envelopment of Herpes Simplex Virus Capsids Analyzed with Dual-Color Fluorescence HSV1(17+). J. Virol. 82: 3109-3124 [Abstract] [Full Text]  
  • Frampton, A. R. Jr., Stolz, D. B., Uchida, H., Goins, W. F., Cohen, J. B., Glorioso, J. C. (2007). Equine Herpesvirus 1 Enters Cells by Two Different Pathways, and Infection Requires the Activation of the Cellular Kinase ROCK1. J. Virol. 81: 10879-10889 [Abstract] [Full Text]  
  • Hambleton, S., Steinberg, S. P., Gershon, M. D., Gershon, A. A. (2007). Cholesterol Dependence of Varicella-Zoster Virion Entry into Target Cells. J. Virol. 81: 7548-7558 [Abstract] [Full Text]  
  • Hoppe, S., Schelhaas, M., Jaeger, V., Liebig, T., Petermann, P., Knebel-Morsdorf, D. (2006). Early herpes simplex virus type 1 infection is dependent on regulated Rac1/Cdc42 signalling in epithelial MDCKII cells. J. Gen. Virol. 87: 3483-3494 [Abstract] [Full Text]  
  • Gianni, T., Forghieri, C., Campadelli-Fiume, G. (2006). The herpesvirus glycoproteins B and H{middle dot}L are sequentially recruited to the receptor-bound gD to effect membrane fusion at virus entry. Proc. Natl. Acad. Sci. USA 103: 14572-14577 [Abstract] [Full Text]  
  • Clement, C., Tiwari, V., Scanlan, P. M., Valyi-Nagy, T., Yue, B. Y.J.T., Shukla, D. (2006). A novel role for phagocytosis-like uptake in herpes simplex virus entry. JCB 174: 1009-1021 [Abstract] [Full Text]  
  • Dohner, K., Radtke, K., Schmidt, S., Sodeik, B. (2006). Eclipse Phase of Herpes Simplex Virus Type 1 Infection: Efficient Dynein-Mediated Capsid Transport without the Small Capsid Protein VP26.. J. Virol. 80: 8211-8224 [Abstract] [Full Text]  
  • Blanchard, E., Belouzard, S., Goueslain, L., Wakita, T., Dubuisson, J., Wychowski, C., Rouille, Y. (2006). Hepatitis C Virus Entry Depends on Clathrin-Mediated Endocytosis. J. Virol. 80: 6964-6972 [Abstract] [Full Text]  
  • Menotti, L., Cerretani, A., Campadelli-Fiume, G. (2006). A Herpes Simplex Virus Recombinant That Exhibits a Single-Chain Antibody to HER2/neu Enters Cells through the Mammary Tumor Receptor, Independently of the gD Receptors.. J. Virol. 80: 5531-5539 [Abstract] [Full Text]  
  • Kwon, H., Bai, Q., Baek, H.-J., Felmet, K., Burton, E. A., Goins, W. F., Cohen, J. B., Glorioso, J. C. (2006). Soluble V Domain of Nectin-1/HveC Enables Entry of Herpes Simplex Virus Type 1 (HSV-1) into HSV-Resistant Cells by Binding to Viral Glycoprotein D. J. Virol. 80: 138-148 [Abstract] [Full Text]  
  • Jin, S., Zhang, B., Weisz, O. A., Montelaro, R. C. (2005). Receptor-Mediated Entry by Equine Infectious Anemia Virus Utilizes a pH-Dependent Endocytic Pathway. J. Virol. 79: 14489-14497 [Abstract] [Full Text]  
  • Taylor, S. L., Moffat, J. F. (2005). Replication of Varicella-Zoster Virus in Human Skin Organ Culture. J. Virol. 79: 11501-11506 [Abstract] [Full Text]  
  • Nicola, A. V., Hou, J., Major, E. O., Straus, S. E. (2005). Herpes Simplex Virus Type 1 Enters Human Epidermal Keratinocytes, but Not Neurons, via a pH-Dependent Endocytic Pathway. J. Virol. 79: 7609-7616 [Abstract] [Full Text]  
  • Milne, R. S. B., Nicola, A. V., Whitbeck, J. C., Eisenberg, R. J., Cohen, G. H. (2005). Glycoprotein D Receptor-Dependent, Low-pH-Independent Endocytic Entry of Herpes Simplex Virus Type 1. J. Virol. 79: 6655-6663 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»