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Journal of Virology, November 2004, p. 12085-12089, Vol. 78, No. 21
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.21.12085-12089.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genetic Interactions between Hepatitis C Virus Replicons

Matthew J. Evans,1,{dagger} Charles M. Rice,2 and Stephen P. Goff1,3*

Integrated Program in Cellular, Molecular, and Biophysical Studies,1 Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, College of Physicians and Surgeons, Columbia University,3 Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, New York2

Received 3 April 2004/ Accepted 16 June 2004

To investigate interactions between hepatitis C virus (HCV) RNA replication complexes, a system was developed to simultaneously select different HCV subgenomic replicons within the same cell. Transcomplementation of defective replicons was not observed, suggesting an isolated and independent nature of the HCV RNA replication complex. In contrast, a high level of competition between replicons was observed, such that the presence and increased fitness of one replicon reduced the capacity of a second one to stably replicate. These results suggest that at least one factor in Huh7 cells required for HCV RNA replication is limiting and saturable.

* Corresponding author. Mailing address: HHSC 1310c, 701 W. 168th St., College of Physicians and Surgeons, Columbia University, New York, NY 10032. Phone: (212) 305-3794. Fax: (212) 305-8692. E-mail: goff{at}cancercenter.columbia.edu.

{dagger} Present address: Center for the Study of Hepatitis C, Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10021.

Journal of Virology, November 2004, p. 12085-12089, Vol. 78, No. 21
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.21.12085-12089.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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