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Journal of Virology, November 2004, p. 12082-12084, Vol. 78, No. 21
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.21.12082-12084.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Low Level of Lytic Replication in a Recombinant Epstein-Barr Virus Carrying an Origin of Replication Devoid of BZLF1-Binding Sites

R. Feederle and H. J. Delecluse*

Cancer Research-UK Institute for Cancer Studies, Department of Pathology, University of Birmingham, Birmingham, United Kingdom

Received 18 March 2004/ Accepted 15 June 2004

Binding of the BZLF1 viral transactivator to Epstein-Barr virus (EBV) oriLyt has been reported to be essential for viral DNA replication. We have constructed a recombinant virus (E2-oriLyt-R) in which the oriLyt BZLF1-binding sites (ZRE) were exchanged against papilloma E2-binding sites. A fusion protein between the BZLF1 protein-transactivating domain and the E2 protein-binding domain was able to reactivate lytic replication in E2-oriLyt-R. However, BZLF1 alone could also induce E2-oriLyt-R, albeit with much lower efficiency. ZRE are therefore important but not absolutely essential cis elements for lytic replication. This shows the importance of recombinants to evaluate viral functions.

* Corresponding author. Present address: German Cancer Research Center, Applied Tumour Virology F-100, Im Neuenheimer Feld 242, D-69120 Heidelberg, Germany. Phone: 49-6221-424870. Fax: 49-6221-424852. E-mail: H.Delecluse{at}dkfz.de.

Journal of Virology, November 2004, p. 12082-12084, Vol. 78, No. 21
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.21.12082-12084.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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