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Journal of Virology, October 2004, p. 11352-11359, Vol. 78, No. 20
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.20.11352-11359.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Lentiviral cDNA Library Employing Lambda Recombination Used To Clone an Inhibitor of Human Immunodeficiency Virus Type 1-Induced Cell Death

Yuji Kawano,1 Takeshi Yoshida,1,2 Kuniko Hieda,1,2 Jun Aoki,1,2 Hiroyuki Miyoshi,3 and Yoshio Koyanagi2*

Department of Virology, Tohoku University Graduate School of Medicine, Sendai,1 Laboratory of Viral Pathogenesis, Institute for Virus Research, Kyoto University, Kyoto,2 BioResource Center, RIKEN Tsukuba Institute, Tsukuba, Japan3

Received 17 November 2003/ Accepted 27 May 2004

Expression cloning technology of cDNAs is a suitable tool for identifying novel functional properties of genes. Here, we generated a lentiviral cDNA library-expressing system for human T cells based on a site-specific recombination system of phage lambda for transferring cDNA libraries with a minimum loss of its complexity. The library-transduced CD4+ T cells were challenged with wild-type human immunodeficiency virus type 1 (HIV-1), and the cells that acquired resistance to HIV-1-induced cytopathic effect (CPE) were selected. From these cells, CD14 was isolated and proved to inhibit the entry of HIV-1 and the HIV-1-induced CPE. This cloning system allows rapid identification of genes encoding novel properties in human T cells and probably other mammalian cells.

* Corresponding author. Mailing address: Laboratory of Viral Pathogenesis, Institute for Virus Research, Kyoto University, 53 Shougoinkawahara cho, Sakyou-ku, Kyoto 606-8507, Japan. Phone: 81-22-717-8210. Fax: 81-75-751-4812. E-mail: ykoyanag{at}virus.kyoto-u.ac.jp.

Journal of Virology, October 2004, p. 11352-11359, Vol. 78, No. 20
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.20.11352-11359.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sato, K., Aoki, J., Misawa, N., Daikoku, E., Sano, K., Tanaka, Y., Koyanagi, Y. (2008). Modulation of Human Immunodeficiency Virus Type 1 Infectivity through Incorporation of Tetraspanin Proteins. J. Virol. 82: 1021-1033 [Abstract] [Full Text]  


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