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Journal of Virology, October 2004, p. 10878-10887, Vol. 78, No. 20
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.20.10878-10887.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

LMP2A Does Not Require Palmitoylation To Localize to Buoyant Complexes or for Function

Rebecca B. Katzman and Richard Longnecker*

Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine, Chicago, Illinois

Received 22 March 2004/ Accepted 26 May 2004

Epstein-Barr virus (EBV) latent membrane protein 2A (LMP2A) is expressed constitutively in lipid rafts in latently infected B lymphocytes. Lipid rafts are membrane microdomains enriched in cholesterol and sphingolipids selective for specific protein association. Lipid rafts have been shown to be necessary for B-cell receptor (BCR) signal transduction. LMP2A prevents BCR recruitment to lipid rafts, thereby abrogating BCR function. As LMP2A is palmitoylated, whether this fatty acid modification is necessary for LMP2A to localize to lipid rafts and for protein function was investigated. LMP2A palmitoylation was confirmed in latently infected B cells. LMP2A was found to be palmitoylated on multiple cysteines only by S acylation. An LMP2A mutant that was not palmitoylated was identified and functioned similar to wild-type LMP2A; unmodified LMP2A localized to lipid rafts, was tyrosine phosphorylated, was associated with LMP2A-associated proteins, was ubiquitinated, and was able to block calcium mobilization following BCR cross-linking. Therefore, palmitoylation of LMP2A is not required for LMP2A targeting to buoyant complexes or for function.

* Corresponding author. Mailing address: Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, IL 60611. Fax: (312) 503-139. E-mail: r-longnecker{at}northwestern.edu.

Journal of Virology, October 2004, p. 10878-10887, Vol. 78, No. 20
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.20.10878-10887.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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