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Journal of Virology, January 2004, p. 995-998, Vol. 78, No. 2
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.2.995-998.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Imparting Temperature Sensitivity and Attenuation in Ferrets to A/Puerto Rico/8/34 Influenza Virus by Transferring the Genetic Signature for Temperature Sensitivity from Cold-Adapted A/Ann Arbor/6/60

Hong Jin,* Helen Zhou, Bin Lu, and George Kemble

MedImmune Vaccines, Inc., Mountain View, California 94043

Received 17 August 2003/ Accepted 2 October 2003

The four temperature-sensitive (ts) loci identified in the PB1 and PB2 gene segments of cold-adapted A/Ann Arbor/6/60 influenza virus, the master donor virus for influenza A virus (MDV-A) FluMist vaccines, were introduced into a divergent A/Puerto Rico/8/34 influenza virus strain. Recombinant A/Puerto Rico/8/34 virus with these four introduced ts loci exhibited both ts and att phenotypes similar to those of MDV-A, which could be used as a donor virus for manufacturing large quantities of inactivated influenza virus vaccine against potential pandemic strains.

* Corresponding author. Mailing address: MedImmune Vaccines, Inc., 297 N. Bernardo Ave., Mountain View, CA 94043. Phone: (650) 919-6587. Fax: (650) 919-2418. E-mail: jinh{at}medimmune.com.

Journal of Virology, January 2004, p. 995-998, Vol. 78, No. 2
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.2.995-998.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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