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Journal of Virology, October 2004, p. 10820-10824, Vol. 78, No. 19
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.19.10820-10824.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification of Basal Promoter and Enhancer Elements in an Untranslated Region of the TT Virus Genome

Tetsuro Suzuki,1* Ryosuke Suzuki,1 Jin Li,1 Minako Hijikata,2 Mami Matsuda,1 Tiang-Cheng Li,1 Yoshiharu Matsuura,3 Shunji Mishiro,4 and Tatsuo Miyamura1

Department of Virology II, National Institute of Infectious Diseases,1 Department of Respiratory Diseases, Research Institute, International Medical Center of Japan, Shinjuku-ku,2 Department of Medical Sciences, Toshiba General Hospital, Shinagawa-ku, Tokyo,4 Research Center for Emerging Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita-shi, Osaka, Japan3

Received 2 April 2004/ Accepted 19 May 2004

The regulation of TT virus (TTV) gene expression was characterized. Transient-transfection assays using reporter constructs revealed that a 113-nucleotide (nt) sequence within the untranslated region, proximal to the transcription initiation site and containing a TATA box motif, has a basal promoter activity. This sequence is well conserved among different TTV genotypes. Upstream stimulating factor bound to a consensus binding motif within this region and positively regulates TTV transcription. Furthermore, a 488-nt region upstream of the basal promoter exhibited enhancer activity, presumably in a cell type-specific manner. This study illustrates some of the mechanisms involved in the transcriptional regulation of TTV.

* Corresponding author. Mailing address: Department of Virology II, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. Phone: (81) 3-5285-1111. Fax: (81) 3-5285-1161. E-mail: tesuzuki{at}nih.go.jp.

Journal of Virology, October 2004, p. 10820-10824, Vol. 78, No. 19
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.19.10820-10824.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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