This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deutsch, E.
Right arrow Articles by Sarid, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deutsch, E.
Right arrow Articles by Sarid, R.

 Previous Article  |  Next Article 

Journal of Virology, September 2004, p. 10187-10192, Vol. 78, No. 18
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.18.10187-10192.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Role of Protein Kinase C {delta} in Reactivation of Kaposi's Sarcoma-Associated Herpesvirus

Einat Deutsch, Adina Cohen, Gila Kazimirsky, Sara Dovrat, Hadara Rubinfeld, Chaya Brodie, and Ronit Sarid*

Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel

Received 30 December 2003/ Accepted 18 May 2004

TPA (12-O-tetradecanoylphorbol-13-acetate), a well-known activator of protein kinase C (PKC), can experimentally induce reactivation of Kaposi's sarcoma-associated herpesvirus (KSHV) in certain latently infected cells. We selectively blocked the activity of PKC isoforms by using GF 109203X or rottlerin and demonstrated that this inhibition largely decreased lytic KSHV reactivation by TPA. Translocation of the PKC{delta} isoform was evident shortly after TPA stimulation. Overexpression of the dominant-negative PKC{delta} mutant supported an essential role for the PKC{delta} isoform in virus reactivation, yet overexpression of PKC{delta} alone was not sufficient to induce lytic reactivation of KSHV, suggesting that additional signaling molecules participate in this pathway.

* Corresponding author. Mailing address: Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 52900, Israel. Phone: 972-3-5317853. Fax: 972-3-5351824. E-mail: saridr{at}mail.biu.ac.il.

Journal of Virology, September 2004, p. 10187-10192, Vol. 78, No. 18
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.18.10187-10192.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lee, H.-H., Chang, S.-S., Lin, S.-J., Chua, H.-H., Tsai, T.-J., Tsai, K., Lo, Y.-C., Chen, H.-C., Tsai, C.-H. (2008). Essential role of PKC{delta} in histone deacetylase inhibitor-induced Epstein-Barr virus reactivation in nasopharyngeal carcinoma cells. J. Gen. Virol. 89: 878-883 [Abstract] [Full Text]  
  • Morris, T. L., Arnold, R. R., Webster-Cyriaque, J. (2007). Signaling Cascades Triggered by Bacterial Metabolic End Products during Reactivation of Kaposi's Sarcoma-Associated Herpesvirus. J. Virol. 81: 6032-6042 [Abstract] [Full Text]  
  • Ford, P. W., Bryan, B. A., Dyson, O. F., Weidner, D. A., Chintalgattu, V., Akula, S. M. (2006). Raf/MEK/ERK signalling triggers reactivation of Kaposi's sarcoma-associated herpesvirus latency.. J. Gen. Virol. 87: 1139-1144 [Abstract] [Full Text]  
  • Cohen, A., Brodie, C., Sarid, R. (2006). An essential role of ERK signalling in TPA-induced reactivation of Kaposi's sarcoma-associated herpesvirus.. J. Gen. Virol. 87: 795-802 [Abstract] [Full Text]  
  • Okhrimenko, H., Lu, W., Xiang, C., Hamburger, N., Kazimirsky, G., Brodie, C. (2005). Protein Kinase C-{varepsilon} Regulates the Apoptosis and Survival of Glioma Cells. Cancer Res. 65: 7301-7309 [Abstract] [Full Text]  
  • Matsumura, S., Fujita, Y., Gomez, E., Tanese, N., Wilson, A. C. (2005). Activation of the Kaposi's Sarcoma-Associated Herpesvirus Major Latency Locus by the Lytic Switch Protein RTA (ORF50). J. Virol. 79: 8493-8505 [Abstract] [Full Text]  
  • Okhrimenko, H., Lu, W., Xiang, C., Ju, D., Blumberg, P. M., Gomel, R., Kazimirsky, G., Brodie, C. (2005). Roles of Tyrosine Phosphorylation and Cleavage of Protein Kinase C{delta} in Its Protective Effect Against Tumor Necrosis Factor-related Apoptosis Inducing Ligand-induced Apoptosis. J. Biol. Chem. 280: 23643-23652 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»