This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Norris, P. J.
Right arrow Articles by Rosenberg, E. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Norris, P. J.
Right arrow Articles by Rosenberg, E. S.

 Previous Article  |  Next Article 

Journal of Virology, August 2004, p. 8844-8851, Vol. 78, No. 16
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.16.8844-8851.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Beyond Help: Direct Effector Functions of Human Immunodeficiency Virus Type 1-Specific CD4+ T Cells

Philip J. Norris,1,2* Howell F. Moffett,2 Otto O. Yang,3 Daniel E. Kaufmann,2 Margaret J. Clark,2 Marylyn M. Addo,2 and Eric S. Rosenberg2

Blood Systems Research Institute, San Francisco, California 94118,1 Partners AIDS Research Center, Massachusetts General Hospital Division of Infectious Diseases and Harvard Medical School, Charlestown, Massachusetts 02129,2 Division of Infectious Diseases/Department of Medicine, Department of Microbiology, Immunology, and Molecular Genetics, AIDS Institute, Geffen School of Medicine, UCLA Medical Center, Los Angeles, California, 900953

Received 19 February 2004/ Accepted 13 April 2004

The immune correlates of protection in human immunodeficiency virus type 1 (HIV-1) infection remain poorly defined, particularly the contribution of CD4+ T cells. Here we explore the effector functions of HIV-1-specific CD4+ T cells. We demonstrate HIV-1 p24-specific CD4+-T-cell cytolytic activity in peripheral blood mononuclear cells directly ex vivo and after enrichment by antigen-specific stimulation. We further show that in a rare long-term nonprogressor, both an HIV-1-specific CD4+-T-cell clone and CD4+ T cells directly ex vivo exert potent suppression of HIV-1 replication. Suppression of viral replication was dependent on cell-cell contact between the effector CD4+ T cells and the target cells. While the antiviral effector activity of CD8+ T cells has been well documented, these results strongly suggest that HIV-1-specific CD4+ T cells are capable of directly contributing to antiviral immunity.

* Corresponding author. Mailing address: Blood Systems Research Institute, 270 Masonic Ave., San Francisco, CA 94118. Phone: (415) 923-5769. Fax: (415) 775-3859. E-mail: pnorris{at}bloodsystems.org.

Journal of Virology, August 2004, p. 8844-8851, Vol. 78, No. 16
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.16.8844-8851.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zheng, N., Fujiwara, M., Ueno, T., Oka, S., Takiguchi, M. (2009). Strong Ability of Nef-Specific CD4+ Cytotoxic T Cells To Suppress Human Immunodeficiency Virus Type 1 (HIV-1) Replication in HIV-1-Infected CD4+ T Cells and Macrophages. J. Virol. 83: 7668-7677 [Abstract] [Full Text]  
  • Sacha, J. B., Giraldo-Vela, J. P., Buechler, M. B., Martins, M. A., Maness, N. J., Chung, C., Wallace, L. T., Leon, E. J., Friedrich, T. C., Wilson, N. A., Hiraoka, A., Watkins, D. I. (2009). Gag- and Nef-specific CD4+ T cells recognize and inhibit SIV replication in infected macrophages early after infection. Proc. Natl. Acad. Sci. USA 106: 9791-9796 [Abstract] [Full Text]  
  • Beal, A. M., Anikeeva, N., Varma, R., Cameron, T. O., Norris, P. J., Dustin, M. L., Sykulev, Y. (2008). Protein Kinase C{theta} Regulates Stability of the Peripheral Adhesion Ring Junction and Contributes to the Sensitivity of Target Cell Lysis by CTL. J. Immunol. 181: 4815-4824 [Abstract] [Full Text]  
  • Li, H., Chien, P. C. Jr., Tuen, M., Visciano, M. L., Cohen, S., Blais, S., Xu, C.-F., Zhang, H.-T., Hioe, C. E. (2008). Identification of an N-Linked Glycosylation in the C4 Region of HIV-1 Envelope gp120 That Is Critical for Recognition of Neighboring CD4 T Cell Epitopes. J. Immunol. 180: 4011-4021 [Abstract] [Full Text]  
  • Kannanganat, S., Ibegbu, C., Chennareddi, L., Robinson, H. L., Amara, R. R. (2007). Multiple-Cytokine-Producing Antiviral CD4 T Cells Are Functionally Superior to Single-Cytokine-Producing Cells. J. Virol. 81: 8468-8476 [Abstract] [Full Text]  
  • Mitra-Kaushik, S., Cruz, J., Stern, L. J., Ennis, F. A., Terajima, M. (2007). Human Cytotoxic CD4+ T Cells Recognize HLA-DR1-Restricted Epitopes on Vaccinia Virus Proteins A24R and D1R Conserved among Poxviruses. J. Immunol. 179: 1303-1312 [Abstract] [Full Text]  
  • Sitati, E. M., Diamond, M. S. (2006). CD4+ T-Cell Responses Are Required for Clearance of West Nile Virus from the Central Nervous System. J. Virol. 80: 12060-12069 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»