This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nomura, R.
Right arrow Articles by Fujimoto, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nomura, R.
Right arrow Articles by Fujimoto, T.

 Previous Article  |  Next Article 

Journal of Virology, August 2004, p. 8701-8708, Vol. 78, No. 16
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.16.8701-8708.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Human Coronavirus 229E Binds to CD13 in Rafts and Enters the Cell through Caveolae

Ryuji Nomura,1* Asuka Kiyota,2 Etsuko Suzaki,2 Katsuko Kataoka,2 Yoshihide Ohe,3 Kaoru Miyamoto,4 Takao Senda,1 and Toyoshi Fujimoto5

Department of Anatomy I, Fujita Health University School of Medicine, Toyoake 470-1192,1 Department of Histology and Cell Biology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima 734-8551,2 Biosignal Research Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371-8512,3 Department of Biochemistry, Fukui Medical University, Matsuoka 910-1193,4 Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan5

Received 14 May 2003/ Accepted 11 May 2004

CD13, a receptor for human coronavirus 229E (HCoV-229E), was identified as a major component of the Triton X-100-resistant membrane microdomain in human fibroblasts. The incubation of living fibroblasts with an anti-CD13 antibody on ice gave punctate labeling that was evenly distributed on the cell surface, but raising the temperature to 37°C before fixation caused aggregation of the labeling. The aggregated labeling of CD13 colocalized with caveolin-1 in most cells. The HCoV-229E virus particle showed a binding and redistribution pattern that was similar to that caused by the anti-CD13 antibody: the virus bound to the cell evenly when incubated on ice but became colocalized with caveolin-1 at 37°C; importantly, the virus also caused sequestration of CD13 to the caveolin-1-positive area. Electron microscopy confirmed that HCoV-229E was localized near or at the orifice of caveolae after incubation at 37°C. The depletion of plasmalemmal cholesterol with methyl ß-cyclodextrin significantly reduced the HCoV-229E redistribution and subsequent infection. A caveolin-1 knockdown by RNA interference also reduced the HCoV-229E infection considerably. The results indicate that HCoV-229E first binds to CD13 in the Triton X-100-resistant microdomain, then clusters CD13 by cross-linking, and thereby reaches the caveolar region before entering cells.

* Corresponding author. Mailing address: Department of Anatomy I, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan. Phone: 81-562-93-2648. Fax: 81-562-93-5904. E-mail: rnomura{at}fujita-hu.ac.jp.

Journal of Virology, August 2004, p. 8701-8708, Vol. 78, No. 16
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.16.8701-8708.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kawase, M., Shirato, K., Matsuyama, S., Taguchi, F. (2009). Protease-Mediated Entry via the Endosome of Human Coronavirus 229E. J. Virol. 83: 712-721 [Abstract] [Full Text]  
  • Regan, A. D., Whittaker, G. R. (2008). Utilization of DC-SIGN for Entry of Feline Coronaviruses into Host Cells. J. Virol. 82: 11992-11996 [Abstract] [Full Text]  
  • Van Hamme, E., Dewerchin, H. L., Cornelissen, E., Verhasselt, B., Nauwynck, H. J. (2008). Clathrin- and caveolae-independent entry of feline infectious peritonitis virus in monocytes depends on dynamin. J. Gen. Virol. 89: 2147-2156 [Abstract] [Full Text]  
  • Pu, Y., Zhang, X. (2008). Mouse Hepatitis Virus Type 2 Enters Cells through a Clathrin-Mediated Endocytic Pathway Independent of Eps15. J. Virol. 82: 8112-8123 [Abstract] [Full Text]  
  • Van Hamme, E., Dewerchin, H. L., Cornelissen, E., Nauwynck, H. J. (2007). Attachment and internalization of feline infectious peritonitis virus in feline blood monocytes and Crandell feline kidney cells. J. Gen. Virol. 88: 2527-2532 [Abstract] [Full Text]  
  • Inoue, Y., Tanaka, N., Tanaka, Y., Inoue, S., Morita, K., Zhuang, M., Hattori, T., Sugamura, K. (2007). Clathrin-Dependent Entry of Severe Acute Respiratory Syndrome Coronavirus into Target Cells Expressing ACE2 with the Cytoplasmic Tail Deleted. J. Virol. 81: 8722-8729 [Abstract] [Full Text]  
  • Beer, C., Pedersen, L. (2007). Matrix Fibronectin Binds Gammaretrovirus and Assists in Entry: New Light on Viral Infections. J. Virol. 81: 8247-8257 [Abstract] [Full Text]  
  • Petrovic, N., Schacke, W., Gahagan, J. R., O'Conor, C. A., Winnicka, B., Conway, R. E., Mina-Osorio, P., Shapiro, L. H. (2007). CD13/APN regulates endothelial invasion and filopodia formation. Blood 110: 142-150 [Abstract] [Full Text]  
  • Miki, T., Takegami, Y., Okawa, K., Muraguchi, T., Noda, M., Takahashi, C. (2007). The Reversion-inducing Cysteine-rich Protein with Kazal Motifs (RECK) Interacts with Membrane Type 1 Matrix Metalloproteinase and CD13/Aminopeptidase N and Modulates Their Endocytic Pathways. J. Biol. Chem. 282: 12341-12352 [Abstract] [Full Text]  
  • Winter, C., Schwegmann-Wessels, C., Cavanagh, D., Neumann, U., Herrler, G. (2006). Sialic acid is a receptor determinant for infection of cells by avian Infectious bronchitis virus.. J. Gen. Virol. 87: 1209-1216 [Abstract] [Full Text]  
  • Alfalah, M., Krahn, M. P., Wetzel, G., von Horsten, S., Wolke, C., Hooper, N., Kalinski, T., Krueger, S., Naim, H. Y., Lendeckel, U. (2006). A Mutation in Aminopeptidase N (CD13) Isolated from a Patient Suffering from Leukemia Leads to an Arrest in the Endoplasmic Reticulum. J. Biol. Chem. 281: 11894-11900 [Abstract] [Full Text]  
  • Assavalapsakul, W., Smith, D. R., Panyim, S. (2006). Identification and Characterization of a Penaeus monodon Lymphoid Cell-Expressed Receptor for the Yellow Head Virus. J. Virol. 80: 262-269 [Abstract] [Full Text]  
  • Beer, C., Andersen, D. S., Rojek, A., Pedersen, L. (2005). Caveola-Dependent Endocytic Entry of Amphotropic Murine Leukemia Virus. J. Virol. 79: 10776-10787 [Abstract] [Full Text]  
  • Choi, K. S., Aizaki, H., Lai, M. M. C. (2005). Murine Coronavirus Requires Lipid Rafts for Virus Entry and Cell-Cell Fusion but Not for Virus Release. J. Virol. 79: 9862-9871 [Abstract] [Full Text]  
  • Lassnig, C., Sanchez, C. M., Egerbacher, M., Walter, I., Majer, S., Kolbe, T., Pallares, P., Enjuanes, L., Muller, M. (2005). From The Cover: Development of a transgenic mouse model susceptible to human coronavirus 229E. Proc. Natl. Acad. Sci. USA 102: 8275-8280 [Abstract] [Full Text]  
  • Kramer, W., Girbig, F., Corsiero, D., Pfenninger, A., Frick, W., Jahne, G., Rhein, M., Wendler, W., Lottspeich, F., Hochleitner, E. O., Orso, E., Schmitz, G. (2005). Aminopeptidase N (CD13) Is a Molecular Target of the Cholesterol Absorption Inhibitor Ezetimibe in the Enterocyte Brush Border Membrane. J. Biol. Chem. 280: 1306-1320 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»