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Journal of Virology, July 2004, p. 7523-7527, Vol. 78, No. 14
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.14.7523-7527.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Inhibition of Severe Acute Respiratory Syndrome Virus Replication by Small Interfering RNAs in Mammalian Cells

Zhi Wang,1,{dagger} Lili Ren,2,{dagger} Xingang Zhao,1 Tao Hung,2 Anming Meng,1 Jianwei Wang,2* and Ye-Guang Chen1*

State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084,1 Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China2

Received 3 December 2003/ Accepted 8 March 2004

Severe acute respiratory syndrome (SARS) is an acute respiratory infectious disease that spread worldwide in early 2003. The cause was determined as a novel coronavirus (CoV), SARS-associated CoV (SARS-CoV), with a single-stranded, plus-sense RNA. To date, no effective specific treatment has been identified. To exploit the possibility of using RNA interference as a therapeutic approach to fight the disease, plasmid-mediated small interfering RNAs (siRNAs) were generated to target the SARS-CoV genome. The expression of siRNAs from two plasmids, which specifically target the viral RNA polymerase, effectively blocked the cytopathic effects of SARS-CoV on Vero cells. These two plasmids also inhibited viral replication as shown by titer assays and by an examination of viral RNA and protein levels. Thus, our results demonstrated the feasibility of developing siRNAs as effective anti-SARS drugs.

* Corresponding author. Mailing address for Ye-Guang Chen: Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China. Phone: 86 10 62795184. Fax: 86 10 62794376. E-mail: ygchen{at}tsinghua.edu.cn. Mailing address for Jianwei Wang: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China. Phone: 86 10 63578244. Fax: 86 10 63529809. E-mail: wangjw28{at}vip.sina.com.

{dagger} Z.W. and L.R. contributed equally to this work.

Journal of Virology, July 2004, p. 7523-7527, Vol. 78, No. 14
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.14.7523-7527.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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