This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohka, S.
Right arrow Articles by Nomoto, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohka, S.
Right arrow Articles by Nomoto, A.

 Previous Article  |  Next Article 

Journal of Virology, July 2004, p. 7186-7198, Vol. 78, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.13.7186-7198.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Receptor (CD155)-Dependent Endocytosis of Poliovirus and Retrograde Axonal Transport of the Endosome

Seii Ohka,1 Norie Matsuda,1 Koujiro Tohyama,2 Toshiyuki Oda,1 Masato Morikawa,1 Shusuke Kuge,1 and Akio Nomoto1*

Department of Microbiology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033,1 The Center for Electron Microscopy and Bio-Imaging Research, Department of Neuroanatomy, Iwate Medical University, Morioka, Iwate 020-8505, Japan2

Received 19 November 2003/ Accepted 18 February 2004

Poliovirus (PV), when injected intramuscularly into the calf, is incorporated into the sciatic nerve and causes an initial paralysis of the inoculated limb in transgenic mice carrying the human PV receptor (hPVR/CD155) gene. Here, we demonstrated by using an immunoelectron microscope that PV particles exist on vesicle structures in nerve terminals of neuromuscular junctions. We also demonstrated in glutathione S-transferase pull-down experiments that the dynein light chain, Tctex-1, interacts directly with the cytoplasmic domain of hPVR. In the axons of differentiated rat PC12 cells transfected with expression vectors for hPVRs, vesicles composed of PV and hPVR{alpha}, as well as a mutant hPVR{alpha} (hPVRM{alpha}) that had a reduced ability to bind Tctex-1, colocalized with Tctex-1. However, vesicles containing PV, dextran, and hPVR{alpha} had only retrograde motion, while those containing PV, dextran, and hPVRM{alpha} had anterograde or retrograde motion. Topical application of the antimicrotubule agent vinblastine to the sciatic nerve reduced the amount of virus transported from the calf to the spinal cord. These results suggest that direct efficient interaction between the cytoplasmic domain and Tctex-1 is essential for the efficient retrograde transport of PV-containing vesicles along microtubules in vivo.

* Corresponding author. Mailing address: Department of Microbiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Phone: 81-3-5841-3407. Fax: 81-3-5841-3374. E-mail: anomoto{at}m.u-tokyo.ac.jp.

Journal of Virology, July 2004, p. 7186-7198, Vol. 78, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.13.7186-7198.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Fujiyuki, T., Matsuzaka, E., Nakaoka, T., Takeuchi, H., Wakamoto, A., Ohka, S., Sekimizu, K., Nomoto, A., Kubo, T. (2009). Distribution of Kakugo Virus and Its Effects on the Gene Expression Profile in the Brain of the Worker Honeybee Apis mellifera L.. J. Virol. 83: 11560-11568 [Abstract] [Full Text]  
  • Ohka, S., Sakai, M., Bohnert, S., Igarashi, H., Deinhardt, K., Schiavo, G., Nomoto, A. (2009). Receptor-Dependent and -Independent Axonal Retrograde Transport of Poliovirus in Motor Neurons. J. Virol. 83: 4995-5004 [Abstract] [Full Text]  
  • Khan, S., Peng, X., Yin, J., Zhang, P., Wimmer, E. (2008). Characterization of the New World Monkey Homologues of Human Poliovirus Receptor CD155. J. Virol. 82: 7167-7179 [Abstract] [Full Text]  
  • Chen, C.-S., Yao, Y.-C., Lin, S.-C., Lee, Y.-P., Wang, Y.-F., Wang, J.-R., Liu, C.-C., Lei, H.-Y., Yu, C.-K. (2007). Retrograde Axonal Transport: a Major Transmission Route of Enterovirus 71 in Mice. J. Virol. 81: 8996-9003 [Abstract] [Full Text]  
  • Lo, K. W.-H., Kogoy, J. M., Pfister, K. K. (2007). The DYNLT3 Light Chain Directly Links Cytoplasmic Dynein to a Spindle Checkpoint Protein, Bub3. J. Biol. Chem. 282: 11205-11212 [Abstract] [Full Text]  
  • Berryman, S., Clark, S., Monaghan, P., Jackson, T. (2005). Early Events in Integrin {alpha}v{beta}6-Mediated Cell Entry of Foot-and-Mouth Disease Virus. J. Virol. 79: 8519-8534 [Abstract] [Full Text]  
  • Egger, D., Bienz, K. (2005). Intracellular location and translocation of silent and active poliovirus replication complexes. J. Gen. Virol. 86: 707-718 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»