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Journal of Virology, July 2004, p. 6758-6765, Vol. 78, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.13.6758-6765.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Geminate Structures of African Cassava Mosaic Virus

Bettina Böttcher,1* Sigrid Unseld,2 Hugo Ceulemans,1,{dagger} Robert B. Russell,1 and Holger Jeske2

Structural and Computational and Biology Programme EMBL, D-69117 Heidelberg ,1 Biologisches Institut, Universität Stuttgart, D-70550 Stuttgart, Germany2

Received 17 December 2003/ Accepted 26 January 2004

Two types of geminate structures were purified from African cassava mosaic geminivirus (ACMV)-infected Nicotiana benthamiana plants and analyzed by electron cryomicroscopy and image reconstruction. After cesium sulfate density gradient centrifugation, they were separated into lighter top (T) and heavier bottom (B) components. T particles comigrated with host proteins, whereas B particles were concentrated in a cesium density typical for complete virions. Both particles were composed of two incomplete icosahedra of 11 capsomers each, but T particles were slightly larger (diameter, 22.5 nm) and less dense in the interior than B particles (diameter, 21.5 nm). T particles were frequently associated with small globules of ~14 nm diameter of unknown origin. The overall structure of ACMV, a begomovirus transmitted by whiteflies, was similar to that of Maize streak virus (MSV), a mastrevirus transmitted by leafhoppers, although the vertices of the icosahedra were less pronounced. Models of ACMV coat proteins based on Satellite tobacco necrosis virus support the exposure of parts of the molecule essential for transmission specificity by whiteflies and provide possible structural explanations for the smaller protrusion of the ACMV capsid relative to MSV. The differences of ACMV and MSV virion shapes are discussed with reference to their different animal vectors.

* Corresponding author. Mailing address: Structural and Computational and Biology Programme EMBL, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. Phone: 49-6221-387-304. Fax: 49-6221-387-306. E-mail: boettcher{at}embl-heidelberg.de.

{dagger} Present address: Division of Biochemistry, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium.

Journal of Virology, July 2004, p. 6758-6765, Vol. 78, No. 13
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.13.6758-6765.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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