This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoneda, M.
Right arrow Articles by Kai, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoneda, M.
Right arrow Articles by Kai, C.

 Previous Article  |  Next Article 

Journal of Virology, June 2004, p. 6676-6681, Vol. 78, No. 12
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.12.6676-6681.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Rinderpest Virus Phosphoprotein Gene Is a Major Determinant of Species-Specific Pathogenicity

Misako Yoneda,1 Ryuichi Miura,1 Thomas Barrett,2 Kyoko Tsukiyama-Kohara,1 and Chieko Kai1*

Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan,1 Institute for Animal Health, Pirbright, Woking, Surrey GU24 ONF, United Kingdom2

Received 3 July 2003/ Accepted 3 March 2004

We previously demonstrated that the rinderpest virus (RPV) hemagglutinin (H) protein plays an important role in determining host range but that other viral proteins are clearly required for full RPV pathogenicity to be manifest in different species. To examine the effects of the RPV nucleocapsid (N) protein and phosphoprotein (P) genes on RPV cross-species pathogenicity, we constructed two new recombinant viruses in which the H and P or the H, N, and P genes of the cattle-derived RPV RBOK vaccine were replaced with those from the rabbit-adapted RPV-Lv strain, which is highly pathogenic in rabbits. The viruses rescued were designated recombinant RPV-lapPH (rRPV-lapPH) and rRPV-lapNPH, respectively. Rabbits inoculated with RPV-Lv become feverish and show leukopenia and a decrease in body weight gain, while clinical signs of infection are never observed in rabbits inoculated with RPV-RBOK or with rRPV-lapH. However, rabbits inoculated with either rRPV-lapPH or rRPV-lapNPH became pyrexic and showed leukopenia. Further, histopathological lesions and high virus titers were clearly observed in the lymphoid tissues from animals infected with rRPV-lapPH or rRPV-lapNPH, although they were not observed in rabbits infected with RPV-RBOK or rRPV-lapH. The clinical, virological, and histopathological signs in rabbits infected with the two new recombinant viruses did not differ significantly; therefore, the RPV P gene was considered to be a key determinant of cross-species pathogenicity.

* Corresponding author. Mailing address: Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Sirokanedai, Minato-ku, Tokyo 108-8639, Japan. Phone: 81-3-5449-5497. Fax: 81-3-5449-5397. E-mail: ckai{at}ims.u-tokyo.ac.jp.

Journal of Virology, June 2004, p. 6676-6681, Vol. 78, No. 12
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.12.6676-6681.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Yoneda, M., Guillaume, V., Ikeda, F., Sakuma, Y., Sato, H., Wild, T. F., Kai, C. (2006). Establishment of a Nipah virus rescue system. Proc. Natl. Acad. Sci. USA 103: 16508-16513 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»