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Journal of Virology, June 2004, p. 5737-5744, Vol. 78, No. 11
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.11.5737-5744.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Features Affecting the Ability of Hepatitis Delta Virus RNAs To Initiate RNA-Directed RNA Synthesis

Severin O. Gudima, Jinhong Chang, and John M. Taylor*

Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111-2497

Received 16 December 2003/ Accepted 23 January 2004

In models of the replication of human hepatitis delta virus (HDV) RNA, it is generally assumed that circular RNAs are the only templates. However, noncircular HDV RNAs are also produced during replication, and it is known that replication can be initiated by transfection with noncircular RNAs. Therefore, strategies were devised to determine the relative ability of different HDV RNA species to initiate RNA replication. One strategy used in vivo intermolecular competition following cotransfection into cells, between two sequence-marked HDV RNA species. Circular RNA templates were found to be at least severalfold more efficient than a dimeric linear template. Unit-length linear species, that is, equivalent to circles opened at different sites, were in most cases but not always of efficiency comparable to that of each other. Greater-than-unit-length linear species were more efficient than unit-length species, presumably because of the increased opportunities for template switching. Genomic linear RNAs were generally of initiation ability comparable to that of antigenomic RNAs. A second strategy measured the ability of initiation to occur on different regions of HDV RNAs that were twice the unit length. In summary, results from these two experimental strategies make clear that linear HDV RNA species, as well as circles, can contribute to the overall process of HDV genome replication. In addition, the results from the two experimental strategies provided information on the impact of template switching during RNA-directed transcription.

* Corresponding author. Mailing address: Fox Chase Cancer Center, 333 Cottman Ave., Philadelphia, PA 19111-2497. Phone: (215) 728-2436. Fax: (215) 728-3105. E-mail: John.Taylor{at}FCCC.edu.

Journal of Virology, June 2004, p. 5737-5744, Vol. 78, No. 11
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.11.5737-5744.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chang, J., Nie, X., Chang, H. E., Han, Z., Taylor, J. (2008). Transcription of Hepatitis Delta Virus RNA by RNA Polymerase II. J. Virol. 82: 1118-1127 [Abstract] [Full Text]  
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  • Hofacker, I. L., Stadler, P. F. (2006). Memory efficient folding algorithms for circular RNA secondary structures. Bioinformatics 22: 1172-1176 [Abstract] [Full Text]  
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