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Journal of Virology, May 2004, p. 5007-5014, Vol. 78, No. 10
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.10.5007-5014.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of gHgL for Attachment of Epstein-Barr Virus to Epithelial Cells Compromises Infection

Corina M. Borza,1,{dagger} Andrew J. Morgan,2 Susan M. Turk,1,{ddagger} and Lindsey M. Hutt-Fletcher1*

School of Biological Sciences, University of Missouri—Kansas City, Kansas City, Missouri,1 Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, Bristol, United Kingdom2

Received 7 October 2003/ Accepted 14 January 2004

Epstein-Barr virus (EBV) is a lymphotropic herpesvirus. However, access to B lymphocytes during primary infection may be facilitated by replication in mucosal epithelial cells. Attachment and penetration of EBV into these two cell types are fundamentally different. Both the distribution of receptors and the cellular origin of the virus impact the efficiency of infection. Epithelial cells potentially offer a wide range of receptors with which virus can interact. We report here on analyses of epithelial cells expressing different combinations of receptors. We find that the stoichiometry of the virus glycoprotein complex that includes gHgL and gp42 affects the use of gHgL not just for entry into epithelial cells but also for attachment. Penetration can be mediated efficiently with either a coreceptor for gp42 or gHgL, but the use of gHgL for attachment as well as penetration greatly compromises its ability to mediate entry.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71130. Phone: (318) 675-4948. Fax: (318) 675-5764. E-mail: lhuttf{at}lsuhsc.edu.

{dagger} Present address: Division of Nephrology, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232.

{ddagger} Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, LA 71130.

Journal of Virology, May 2004, p. 5007-5014, Vol. 78, No. 10
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.10.5007-5014.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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