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Journal of Virology, January 2004, p. 513-519, Vol. 78, No. 1
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.1.513-519.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Chromosome-Protein Interactions in Polyomavirus Virions

Mariarosaria Carbone,1 Giuseppe Ascione,1 Silvia Chichiarelli,2 Marie-Isabelle Garcia,1 Margherita Eufemi,2 and Paolo Amati1*

Dipartimento di Biotecnologie Cellulari ed Ematologia, Sezione di Genetica Molecolare,1 Dipartimento di Scienze Biochimiche "A. Rossi Fanelli," Università di Roma "La Sapienza," 00161 Rome, Italy2

Received 23 June 2003/ Accepted 17 September 2003

In this work, we sought to determine whether the components of the murine polyomavirus capsid establish specific interactions with the minichromosome encapsidated into the mature viral particles by using the cis-diamminedichloroplatinum(II) cross-linking reagent. Our data indicated that VP1, but not minor capsid proteins, interacts with the viral genome in vivo. In addition, semiquantitative PCR assays performed on cross-linked DNA complexes revealed that VP1 binds to all regions of the viral genome but significantly more to the regulatory region. The implications of such an interaction for viral infectivity are discussed.

* Corresponding author. Mailing address: Dipartimento di Biotecnologie Cellulari ed Ematologia, Sezione di Genetica Molecolare, Università di Roma "La Sapienza," Viale Regina Elena 324, 00161 Rome, Italy. Phone: 3906490393. Fax: 39064462891. E-mail: amati{at}bce.uniroma1.it.

Journal of Virology, January 2004, p. 513-519, Vol. 78, No. 1
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.1.513-519.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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