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Journal of Virology, January 2004, p. 314-319, Vol. 78, No. 1
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.1.314-319.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genotyping of Porcine Endogenous Retroviruses from a Family of Miniature Swine

Gary Quinn,1* James Wood,1 Kristen Suling,1 Scott Arn,2 David H. Sachs,2 Henk-Jan Schuurman,1 and Clive Patience1

Immerge BioTherapeutics Inc., Cambridge, Massachusetts 02139,1 Transplantation Biology Research Center, Massachusetts General Hospital, Charlestown, Massachusetts 021292

Received 12 August 2003/ Accepted 8 September 2003

The identification of animals in an inbred miniature swine herd that consistently fail to produce replication- competent humantropic porcine endogenous retrovirus (PERV) has prompted studies on the biology of PERV in transmitter and nontransmitter animals. We analyzed PERV RNA transcript profiles in a family of inbred miniature swine (SLAd/d haplotype) in which individual members differed in their capacity to generate humantropic and ecotropic (i.e., pigtropic) virus. We identified unique HaeIII and HpaII gag restriction fragment length polymorphism (RFLP) profiles resulting from single nucleotide polymorphisms in blood cells; these were found only in animals that produced humantropic PERV. These HaeIII and HpaII gag RFLP profiles proved to be components of humantropic PERV as they were transmitted to 293 human target cells in vitro. The humantropic HaeIII and HpaII gag RFLP genotypes in the family of study were not present in other miniature swine in the herd that produced humantropic PERV, indicating that these RFLP profiles relate specifically to this family's lineage.

* Corresponding author. Mailing address: Immerge BioTherapeutics Inc., 300 Technology Sq., Cambridge, MA 02139. Phone: (617) 241-5565. Fax: (617) 241-0539. E-mail: gary.quinn{at}immergebt.com.

Journal of Virology, January 2004, p. 314-319, Vol. 78, No. 1
0022-538X/04/$08.00+0     DOI: 10.1128/JVI.78.1.314-319.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Preuss, T., Fischer, N., Boller, K., Tonjes, R. R. (2006). Isolation and characterization of an infectious replication-competent molecular clone of ecotropic porcine endogenous retrovirus class C.. J. Virol. 80: 10258-10261 [Abstract] [Full Text]  
  • Harrison, I., Takeuchi, Y., Bartosch, B., Stoye, J. P. (2004). Determinants of High Titer in Recombinant Porcine Endogenous Retroviruses. J. Virol. 78: 13871-13879 [Abstract] [Full Text]  


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