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Journal of Virology, April 2003, p. 4401-4408, Vol. 77, No. 7
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.7.4401-4408.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Cytoskeletal Requirements for Hepatitis C Virus (HCV) RNA Synthesis in the HCV Replicon Cell Culture System

Anne G. Bost, Daryl Venable, Lifei Liu, and Beverly A. Heinz*

Infectious Diseases Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285

Received 15 July 2002/ Accepted 23 December 2002

Hepatitis C virus (HCV) induces microtubule aggregates in infected hepatocytes. To determine if cytoskeletal elements are important for HCV RNA synthesis, we examined the effect of cytoskeleton inhibitors on HCV replicon transcription in Huh7 cells. The data demonstrate that HCV replication complex-mediated RNA synthesis requires microtubule and actin polymerization.

* Corresponding author. Mailing address: Infectious Diseases Research, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN 46285-0438. Phone: (317) 433-6933. Fax: (317) 276-1743. E-mail: heinz_beverly_a{at}lilly.com.

Journal of Virology, April 2003, p. 4401-4408, Vol. 77, No. 7
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.7.4401-4408.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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