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Journal of Virology, February 2003, p. 2600-2606, Vol. 77, No. 4
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.4.2600-2606.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A Functional Epitope Determinant on Domain III of the Japanese Encephalitis Virus Envelope Protein Interacted with Neutralizing-Antibody Combining Sites

Cheng-Wen Lin and Suh-Chin Wu*

Institute of Biotechnology and Department of Life Science, National Tsing-Hua University, Hsinchu 30013, Taiwan

Received 12 September 2002/ Accepted 13 November 2002

The envelope (E) protein of Japanese encephalitis virus (JEV) is associated with viral binding to cellular receptors, membrane fusion, and the induction of protective neutralizing-antibody responses in hosts. Most previous studies have not provided detailed molecular information about the spatial configuration of the functional epitopes on domain III of the E protein. Here site-directed mutagenesis was performed to demonstrate that the functional epitope determinants at Ser331 and Asp332 on domain III of the JEV E protein interacted with neutralizing monoclonal antibody (MAb) E3.3. Bacterial expression of the recombinant Fab E3.3 confirmed the molecular interactions of Arg94 in complementary determining region H3 with Ser331 and Asp332 on domain III. This study elucidates the detailed molecular structures of the neutralizing epitope determinants on JEV domain III, which can provide useful information for designing new vaccines.

* Corresponding author. Mailing address: Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan. Phone: 886-3-5742906. Fax: 886-3-5715934. E-mail: scwu{at}life.nthu.edu.tw.

Journal of Virology, February 2003, p. 2600-2606, Vol. 77, No. 4
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.4.2600-2606.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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