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Journal of Virology, February 2003, p. 2377-2384, Vol. 77, No. 4
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.4.2377-2384.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The 64-Kilodalton Capsid Protein Homolog of Beet Yellows Virus Is Required for Assembly of Virion Tails

Alberto J. Napuli,1 Dina V. Alzhanova,1 Catalin E. Doneanu,2 Douglas F. Barofsky,2 Eugene V. Koonin,3 and Valerian V. Dolja1*

Department of Botany and Plant Pathology,1 Department of Chemistry, Oregon State University, Corvallis, Oregon 97331,2 National Center for Biotechnology Information, National Institutes of Health, Bethesda, Maryland 208943

Received 9 September 2002/ Accepted 14 November 2002

The filamentous virion of the closterovirus Beet yellows virus (BYV) consists of a long body formed by the major capsid protein (CP) and a short tail composed of the minor capsid protein (CPm) and the virus-encoded Hsp70 homolog. By using nano-liquid chromatography-tandem mass spectrometry and biochemical analyses, we show here that the BYV 64-kDa protein (p64) is the fourth integral component of BYV virions. The N-terminal domain of p64 is exposed at the virion surface and is accessible to antibodies and mild trypsin digestion. In contrast, the C-terminal domain is embedded in the virion and is inaccessible to antibodies or trypsin. The C-terminal domain of p64 is shown to be homologous to CP and CPm. Mutation of the signature motifs of capsid proteins of filamentous RNA viruses in p64 results in the formation of tailless virions, which are unable to move from cell to cell. These results reveal the dual function of p64 in tail assembly and BYV motility and support the concept of the virion tail as a specialized device for BYV cell-to-cell movement.

* Corresponding author. Mailing address: Department of Botany and Plant Pathology, Oregon State University, Cordley Hall 2082, Corvallis, OR 97331. Phone: (541) 737-5472. Fax: (541) 737-3573. E-mail: doljav{at}science.oregonstate.edu.

Journal of Virology, February 2003, p. 2377-2384, Vol. 77, No. 4
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.4.2377-2384.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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