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Journal of Virology, February 2003, p. 2243-2246, Vol. 77, No. 3
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.3.2243-2246.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Foot-and-Mouth Disease Virus cis-Acting Replication Element (cre) Can Be Complemented in trans within Infected Cells

Laurence Tiley,{dagger} Andrew M. Q. King, and Graham J. Belsham*

Institute for Animal Health, Pirbright, Woking, Surrey GU24 ONF, United Kingdom

Received 2 July 2002/ Accepted 1 November 2002

A temperature-sensitive (ts) mutation was identified within the 5'-untranslated region of foot-and-mouth disease virus (FMDV) RNA. The mutation destabilizes a stem-loop structure recently identified as a cis-acting replication element (cre). Genetic analyses indicated that the ts defect in virus replication could be complemented. Thus, the FMDV cre can function in trans. It is suggested that the cre be renamed a 3B-uridylylation site (bus).

* Corresponding author. Mailing address: Institute for Animal Health, Pirbright, Woking, Surrey GU24 ONF, United Kingdom. Phone: 44 1483 232441. Fax: 44 1483 232448. E-mail: graham.belsham{at}bbsrc.ac.uk.

{dagger} Present address: University of Cambridge, Department of Clinical Veterinary Medicine, Cambridge CB3 0ES, United Kingdom.

Journal of Virology, February 2003, p. 2243-2246, Vol. 77, No. 3
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.3.2243-2246.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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