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Journal of Virology, December 2003, p. 13156-13160, Vol. 77, No. 24
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.24.13156-13160.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Immunoprotective Activity and Safety of a Respiratory Syncytial Virus Vaccine: Mucosal Delivery of Fusion Glycoprotein with a CpG Oligodeoxynucleotide Adjuvant

Gregory A. Prince,1* James J. Mond,2 David D. Porter,3 Kevin C. Yim,1 Steve J. Lan,1 and Dennis M. Klinman4

Virion Systems, Inc., Rockville, Maryland 20850,1 Biosynexus, Inc., Gaithersburg, Maryland 20877,2 Department of Pathology and Laboratory Medicine, University of California School of Medicine, Los Angeles, California 90095,3 Section of Retroviral Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 208924

Received 29 May 2003/ Accepted 10 September 2003

CpG oligodeoxynucleotides (ODN) were identified that stimulated immunoglobulin production and cell proliferation in cotton rat cells in vitro. Three of these ODN were used as a mucosal adjuvant in the noses of cotton rats immunized via this route with respiratory syncytial virus fusion (F) protein. The CpG ODN markedly increased the cotton rat humoral neutralizing-antibody response to respiratory syncytial virus. Such immunized animals had a marked reduction in the production of infectious virus after a live-virus challenge. Animals immunized with the combination of F protein and CpG developed enhanced pulmonary pathology consisting of alveolitis and interstitial pneumonitis after a live-virus challenge. Similar enhanced disease has been seen in cotton rats and children immunized with formalin-inactivated respiratory syncytial virus.

* Corresponding author. Mailing address: Virion Systems, Inc., 9610 Medical Center Dr., Suite 100, Rockville, MD 20850-3347. Phone: (301) 309-1844. Fax: (301) 309-0471. E-mail: gprince{at}erols.com.

Journal of Virology, December 2003, p. 13156-13160, Vol. 77, No. 24
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.24.13156-13160.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Mapletoft, J. W., Oumouna, M., Kovacs-Nolan, J., Latimer, L., Mutwiri, G., Babiuk, L. A., van Drunen Littel-van den Hurk, S. (2008). Intranasal immunization of mice with a formalin-inactivated bovine respiratory syncytial virus vaccine co-formulated with CpG oligodeoxynucleotides and polyphosphazenes results in enhanced protection. J. Gen. Virol. 89: 250-260 [Abstract] [Full Text]  
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