This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kong, W.-P.
Right arrow Articles by Nabel, G. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kong, W.-P.
Right arrow Articles by Nabel, G. J.

 Previous Article  |  Next Article 

Journal of Virology, December 2003, p. 12764-12772, Vol. 77, No. 23
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.23.12764-12772.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Immunogenicity of Multiple Gene and Clade Human Immunodeficiency Virus Type 1 DNA Vaccines

Wing-Pui Kong,1 Yue Huang,1 Zhi-Yong Yang,1 Bimal K. Chakrabarti,1 Zoe Moodie,2 and Gary J. Nabel1*

Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-3005,1 Statistical Center for HIV/AIDS Research and Prevention, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-10242

Received 16 April 2003/ Accepted 26 August 2003

The ability to elicit an immune response to a spectrum of human immunodeficiency virus type 1 (HIV-1) gene products from divergent strains is a desirable feature of an AIDS vaccine. In this study, we examined combinations of plasmids expressing multiple HIV-1 genes from different clades for their ability to elicit humoral and cellular immune responses in mice. Immunization with a modified Env, gp145{Delta}CFI, in combination with a Gag-Pol-Nef fusion protein plasmid elicited similar CD4+ and CD8+ cellular responses to immunization with either vector alone. Further, when mice were immunized with a mixture of Env from three clades, A, B, and C, together with Gag-Pol-Nef, the overall potency and balance of CD4+- and CD8+-T-cell responses to all viral antigens were similar, with only minor differences noted. In addition, plasmid mixtures elicited antibody responses comparable to those from individual inoculations. These findings suggest that a multigene and multiclade vaccine, including components from A, B, and C Env and Gag-Pol-Nef, can broaden antiviral immune responses without immune interference. Such combinations of immunogens may help to address concerns about viral genetic diversity for a prospective HIV-1 vaccine.

* Corresponding author. Mailing address: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bldg. 40, Room 4502, MSC 3005, 40 Convent Dr., Bethesda, MD 20892-3005. Phone: (301) 496-1852. Fax: (301) 480-0274. E-mail: gnabel{at}nih.gov.

Journal of Virology, December 2003, p. 12764-12772, Vol. 77, No. 23
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.23.12764-12772.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kong, W.-P., Wu, L., Wallstrom, T. C., Fischer, W., Yang, Z.-Y., Ko, S.-Y., Letvin, N. L., Haynes, B. F., Hahn, B. H., Korber, B., Nabel, G. J. (2009). Expanded Breadth of the T-Cell Response to Mosaic Human Immunodeficiency Virus Type 1 Envelope DNA Vaccination. J. Virol. 83: 2201-2215 [Abstract] [Full Text]  
  • Schoenly, K. A., Weiner, D. B. (2008). Human Immunodeficiency Virus Type 1 Vaccine Development: Recent Advances in the Cytotoxic T-Lymphocyte Platform "Spotty Business". J. Virol. 82: 3166-3180 [Full Text]  
  • Liang, R., van den Hurk, J. V., Landi, A., Lawman, Z., Deregt, D., Townsend, H., Babiuk, L. A., van Drunen Littel-van den Hurk, S. (2008). DNA prime protein boost strategies protect cattle from bovine viral diarrhea virus type 2 challenge. J. Gen. Virol. 89: 453-466 [Abstract] [Full Text]  
  • Kong, W.-p., Hood, C., Yang, Z.-y., Wei, C.-J., Xu, L., Garcia-Sastre, A., Tumpey, T. M., Nabel, G. J. (2006). Protective immunity to lethal challenge of the 1918 pandemic influenza virus by vaccination. Proc. Natl. Acad. Sci. USA 103: 15987-15991 [Abstract] [Full Text]  
  • Sathaliyawala, T., Rao, M., Maclean, D. M., Birx, D. L., Alving, C. R., Rao, V. B. (2006). Assembly of Human Immunodeficiency Virus (HIV) Antigens on Bacteriophage T4: a Novel In Vitro Approach To Construct Multicomponent HIV Vaccines.. J. Virol. 80: 7688-7698 [Abstract] [Full Text]  
  • Weaver, E. A., Lu, Z., Camacho, Z. T., Moukdar, F., Liao, H.-X., Ma, B.-J., Muldoon, M., Theiler, J., Nabel, G. J., Letvin, N. L., Korber, B. T., Hahn, B. H., Haynes, B. F., Gao, F. (2006). Cross-Subtype T-Cell Immune Responses Induced by a Human Immunodeficiency Virus Type 1 Group M Consensus Env Immunogen. J. Virol. 80: 6745-6756 [Abstract] [Full Text]  
  • Sheets, R. L., Stein, J., Manetz, T. S., Duffy, C., Nason, M., Andrews, C., Kong, W.-P., Nabel, G. J., Gomez, P. L. (2006). Biodistribution of DNA Plasmid Vaccines against HIV-1, Ebola, Severe Acute Respiratory Syndrome, or West Nile Virus Is Similar, without Integration, despite Differing Plasmid Backbones or Gene Inserts. Toxicol Sci 91: 610-619 [Abstract] [Full Text]  
  • Burgers, W. A., van Harmelen, J. H., Shephard, E., Adams, C., Mgwebi, T., Bourn, W., Hanke, T., Williamson, A.-L., Williamson, C. (2006). Design and preclinical evaluation of a multigene human immunodeficiency virus type 1 subtype C DNA vaccine for clinical trial. J. Gen. Virol. 87: 399-410 [Abstract] [Full Text]  
  • Kong, W.-p., Xu, L., Stadler, K., Ulmer, J. B., Abrignani, S., Rappuoli, R., Nabel, G. J. (2005). Modulation of the Immune Response to the Severe Acute Respiratory Syndrome Spike Glycoprotein by Gene-Based and Inactivated Virus Immunization. J. Virol. 79: 13915-13923 [Abstract] [Full Text]  
  • Song, M. K., Vindurampulle, C. J., Capozzo, A. V. E., Ulmer, J., Polo, J. M., Pasetti, M. F., Barry, E. M., Levine, M. M. (2005). Characterization of Immune Responses Induced by Intramuscular Vaccination with DNA Vaccines Encoding Measles Virus Hemagglutinin and/or Fusion Proteins. J. Virol. 79: 9854-9861 [Abstract] [Full Text]  
  • Barouch, D. H., Yang, Z.-y., Kong, W.-p., Korioth-Schmitz, B., Sumida, S. M., Truitt, D. M., Kishko, M. G., Arthur, J. C., Miura, A., Mascola, J. R., Letvin, N. L., Nabel, G. J. (2005). A Human T-Cell Leukemia Virus Type 1 Regulatory Element Enhances the Immunogenicity of Human Immunodeficiency Virus Type 1 DNA Vaccines in Mice and Nonhuman Primates. J. Virol. 79: 8828-8834 [Abstract] [Full Text]  
  • Wu, L., Kong, W.-p., Nabel, G. J. (2005). Enhanced Breadth of CD4 T-Cell Immunity by DNA Prime and Adenovirus Boost Immunization to Human Immunodeficiency Virus Env and Gag Immunogens. J. Virol. 79: 8024-8031 [Abstract] [Full Text]  
  • Brown, B. K., Darden, J. M., Tovanabutra, S., Oblander, T., Frost, J., Sanders-Buell, E., de Souza, M. S., Birx, D. L., McCutchan, F. E., Polonis, V. R. (2005). Biologic and Genetic Characterization of a Panel of 60 Human Immunodeficiency Virus Type 1 Isolates, Representing Clades A, B, C, D, CRF01_AE, and CRF02_AG, for the Development and Assessment of Candidate Vaccines. J. Virol. 79: 6089-6101 [Abstract] [Full Text]  
  • Seaman, M. S., Xu, L., Beaudry, K., Martin, K. L., Beddall, M. H., Miura, A., Sambor, A., Chakrabarti, B. K., Huang, Y., Bailer, R., Koup, R. A., Mascola, J. R., Nabel, G. J., Letvin, N. L. (2005). Multiclade Human Immunodeficiency Virus Type 1 Envelope Immunogens Elicit Broad Cellular and Humoral Immunity in Rhesus Monkeys. J. Virol. 79: 2956-2963 [Abstract] [Full Text]  
  • Richardson, B. A., Overbaugh, J. (2005). Basic Statistical Considerations in Virological Experiments. J. Virol. 79: 669-676 [Full Text]  
  • Akahata, W., Yang, Z.-y., Nabel, G. J. (2005). Comparative Immunogenicity of Human Immunodeficiency Virus Particles and Corresponding Polypeptides in a DNA Vaccine. J. Virol. 79: 626-631 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»