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Journal of Virology, November 2003, p. 12132-12139, Vol. 77, No. 22
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.22.12132-12139.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Intracellularly Expressed Single-Domain Antibody against p15 Matrix Protein Prevents the Production of Porcine Retroviruses

Sylvia Dekker,¹ Wendy Toussaint,¹ George Panayotou,² Ton de Wit,¹ Pim Visser,¹ Frank Grosveld,¹ and Dubravka Drabek^1*

Department of Cell Biology and Genetics, Faculty of Medicine, Erasmus Medical Center Rotterdam, 3000 DR Rotterdam, The Netherlands,¹ Biomedical Sciences Research Center Alexander Fleming, Varkiza 16602, Greece²

Received 7 May 2003/ Accepted 11 August 2003

The presence of porcine endogenous retroviruses presents a potential risk of transmission of infectious diseases (xenozoonosis) if tissues and organs from genetically modified pigs are to be used in xenotransplantation. Here, we report that intracellular expression of a llama single-domain antibody against p15, the matrix domain protein of the porcine endogenous retrovirus Gag polyprotein, blocks retrovirus production, providing the possibility of eliminating the risk of infection in xenotransplantation.

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* Corresponding author. Mailing address: Department of Cell Biology and Genetics, Faculty of Medicine, Erasmus MC Rotterdam, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands. Phone: 3110 4087454. Fax: 3110 4089468. E-mail: d.drabek{at}erasmusmc.nl.

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Journal of Virology, November 2003, p. 12132-12139, Vol. 77, No. 22
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.22.12132-12139.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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