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Journal of Virology, October 2003, p. 11279-11283, Vol. 77, No. 20
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.20.11279-11283.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Dissection of the Vaccinia Virus I7L Core Protein Proteinase

Chelsea M. Byrd,1 Tove' C. Bolken,2 and Dennis E. Hruby2,3*

Molecular and Cellular Biology Program,1 Department of Microbiology, Oregon State University, Corvallis, Oregon 97331,3 SIGA Technologies, Inc., Corvallis, Oregon 973332

Received 27 May 2003/ Accepted 23 July 2003

The vaccinia virus I7L gene product is predicted to be a cysteine proteinase and is demonstrated in this study to be responsible for cleavage of each of the three major core protein precursors (P4a, P4b, and P25K) in vivo. Mutagenesis of the putative catalytic triad of I7L or of the cleavage sites in the core protein precursors inhibits processing. A truncated protein lost the ability to cleave the core protein precursors.

* Corresponding author. Mailing address: Oregon State University, 220 Nash Hall, Corvallis, OR 97331. Phone: (541) 737-1849. Fax: (541) 737-2440. E-mail: hrubyd{at}bcc.orst.edu.

Journal of Virology, October 2003, p. 11279-11283, Vol. 77, No. 20
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.20.11279-11283.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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