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Journal of Virology, January 2003, p. 1633-1637, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1633-1637.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Transcription Initiation Activity of Adenovirus Left-End Sequence in Adenovirus Vectors with E1 Deleted

Masato Yamamoto,* Julia Davydova, Koichi Takayama, Ramon Alemany, and David T. Curiel

Division of Human Gene Therapy, Departments of Medicine, Pathology and Surgery, and the Gene Therapy Center, University of Alabama at Birmingham, Birmingham, Alabama 35294-2172

Received 12 April 2002/ Accepted 8 October 2002

We analyzed the transcription initiation activity of the left-end sequence (first 342 bp) of the adenovirus genome in the context of an adenovirus vector with E1 deleted in in vitro and in vivo gene transfer models. While nucleotide sequences 1 to 190 and 1 to 342 showed strong activity in three out of three lung cancer cell lines, nucleotide sequence 1 to 103 showed limited activity in H358, cells which show characteristics of type 2 alveolar cells. In vivo, the transcription initiation activities of nucleotide sequence 1 to 103 in the liver and the lung were minimal, while nucleotide sequences 1 to 190 and 1 to 342 showed strong activity comparable to that of the cytomegalovirus promoter. Further understanding of the transcription initiation activity of the left-end sequence of the adenovirus genome should lead to optimization of adenovirus vectors.

* Corresponding author. Mailing address: Division of Gene Therapy, University of Alabama at Birmingham, BMR2-408, 901 19th St. South, Birmingham, AL 35294-2172. Phone: (205) 975-0172. Fax: (205) 975-8565. E-mail: Masato.Yamamoto{at}ccc.uab.edu.

Journal of Virology, January 2003, p. 1633-1637, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1633-1637.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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