This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dolcetti, R.
Right arrow Articles by Tognon, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dolcetti, R.
Right arrow Articles by Tognon, M.

 Previous Article  |  Next Article 

Journal of Virology, January 2003, p. 1595-1597, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1595-1597.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Simian Virus 40 Sequences in Human Lymphoblastoid B-Cell Lines

Riccardo Dolcetti,1 Fernanda Martini,2 Michele Quaia,1 Annunziata Gloghini,3 Beatrice Vignocchi,2 Roberta Cariati,1 Marcella Martinelli,2 Antonino Carbone,3 Mauro Boiocchi,1 and Mauro Tognon2,4*

Divisions of Experimental Oncology,1 Pathology, Centro di Riferimento Oncologico, IRCCS, 33081 Aviano (Pordenone),3 Department of Morphology and Embryology, Section of Histology and Embryology,2 Center of Biotechnology, University of Ferrara, 44100 Ferrara, Italy4

Received 19 July 2002/ Accepted 17 October 2002

Human Epstein-Barr virus-immortalized lymphoblastoid B-cell lines tested positive by PCR for simian virus 40 (SV40) DNA (22 of 42 cell lines, 52.3%). B lymphocytes or tissues from which B-cell lines derived were also SV40 positive. In situ hybridization showed that SV40 DNA was present in the nucleus of a small fraction (1/250) of cells. SV40 T-antigen mRNA was detected by reverse transcription-PCR. Lymphoblastoid B-cell lines (n = 4) infected with SV40 remained SV40 positive for 4 to 6 months. SV40-positive B-cell lines were more tumorigenic in SCID mice than were SV40-negative cell lines (4 of 5 [80%] SV40-positive cell lines versus 2 of 4 [50%] SV40-negative cell lines). These results suggest that SV40 may play a role in the early phases of human lymphomagenesis.

* Corresponding author. Mailing address: Department of Morphology and Embryology, School of Medicine, University of Ferrara, Via Fossato di Mortara 64/B, 44100 Ferrara, Italy. Phone: 39 0532 291538. Fax: 39 0532 291533. E-mail: tgm{at}unife.it.

Journal of Virology, January 2003, p. 1595-1597, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1595-1597.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»