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Journal of Virology, January 2003, p. 1589-1594, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1589-1594.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Proline 78 Is Crucial for Human Immunodeficiency Virus Type 1 Nef To Down-Regulate Class I Human Leukocyte Antigen

Takeshi Yamada,1,{dagger} Naotoshi Kaji,1,2 Takashi Odawara,1 Joe Chiba,2 Aikichi Iwamoto,1,3 and Yoshihiro Kitamura1*

Division of Infectious Diseases, Advanced Clinical Research Center,1 Department of Infectious Disease and Applied Immunology, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639,3 Department of Biological Science and Technology, Science University of Tokyo, Chiba 278-8510, Japan2

Received 18 April 2002/ Accepted 4 October 2002

Human immunodeficiency virus type 1 Nef down-regulates human leukocyte antigen class I (HLA-I) in T lymphocytes, and the down-regulation involves the Nef proline-rich domain (PRD) containing four prolines at positions 69, 72, 75, and 78. We used a Sendai virus vector with nef and examined regulation by Nef of HLA-I and CD4 in suspension cultures of cells such as T lymphocytes. Analyses of a series of PRD substitution mutants indicated that, because the substitution of Pro78 with Ala abolished down-regulation of HLA-I but not of CD4, Pro78 is important for HLA-I down-regulation in T lymphocytes.

* Corresponding author. Mailing address: Division of Infectious Diseases, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. Phone: 81-3-5449-5336. Fax: 81-3-5449-5427. E-mail: yochan{at}ims.u-tokyo.ac.jp.

{dagger} Present address: Department of Microbiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.

Journal of Virology, January 2003, p. 1589-1594, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1589-1594.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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