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Journal of Virology, January 2003, p. 1584-1588, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1584-1588.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Common Sites of Retroviral Integration in Mouse Hematopoietic Tumors Identified by High-Throughput, Single Nucleotide Polymorphism-Based Mapping and Bacterial Artificial Chromosome Hybridization

Haifa Shen,1,{dagger} Takeshi Suzuki,1 David J. Munroe,2 Claudia Stewart,2 Lynn Rasmussen,2 Debra J. Gilbert,1 Nancy A. Jenkins,1 and Neal G. Copeland1*

Mouse Cancer Genetics Program, National Cancer Institute-Frederick,1 Laboratory of Molecular Technology, SAIC-Frederick, Frederick, Maryland 217022

Received 9 August 2002/ Accepted 19 September 2002

Retroviral insertional mutagenesis in mouse hematopoietic tumors provides a powerful cancer gene discovery tool. Here, we describe a high-throughput, single nucleotide polymorphism (SNP)-based method, for mapping retroviral integration sites cloned from mouse tumors, and a bacterial artificial chromosome (BAC) hybridization method, for localizing these retroviral integration sites to common sites of retroviral integration (CISs). Several new CISs were identified, including one CIS that mapped near Notch1, a gene that has been causally associated with human T-cell tumors. This mapping method is applicable to many different species, including ones where few genetic markers and little genomic sequence information are available. It can also be used to map endogenous proviruses.

* Corresponding author. Mailing address: Mouse Cancer Genetics Program, National Cancer Institute-Frederick, Frederick, MD 21702. Phone: (301) 846-1260. Fax: (301) 846-6666. E-mail: copeland{at}ncifcrf.gov.

{dagger} Present address: Lexicon Genetics, The Woodlands, TX 77381.

Journal of Virology, January 2003, p. 1584-1588, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1584-1588.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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