This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Salanueva, I. J.
Right arrow Articles by Risco, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salanueva, I. J.
Right arrow Articles by Risco, C.

 Previous Article  |  Next Article 

Journal of Virology, January 2003, p. 1368-1381, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1368-1381.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Polymorphism and Structural Maturation of Bunyamwera Virus in Golgi and Post-Golgi Compartments

Iñigo J. Salanueva,1 Reyes R. Novoa,1 Pilar Cabezas,1 Carmen López-Iglesias,2 José L. Carrascosa,1 Richard M. Elliott,3 and Cristina Risco1*

Department of Macromolecular Structure, Centro Nacional de Biotecnología, CSIC, Cantoblanco, 28049 Madrid,1 Universitat de Barcelona, Serveis Cientificotècnics, Unitats de Reconeixement Molecular in situ i Genòmica, 08028 Barcelona, Spain,2 Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G11 5JR, United Kingdom3

Received 14 June 2002/ Accepted 10 October 2002

The Golgi apparatus is the assembly site for a number of complex enveloped viruses. Using high-preservation methods for electron microscopy, we have detected two previously unknown maturation steps in the morphogenesis of Bunyamwera virus in BHK-21 cells. The first maturation takes place inside the Golgi stack, where annular immature particles transform into dense, compact structures. Megalomicin, a drug that disrupts the trans side of the Golgi complex, reversibly blocks transformation, showing that a functional trans-Golgi is needed for maturation. The second structural change seems to take place during the egress of viral particles from cells, when a coat of round-shaped spikes becomes evident. A fourth viral assembly was detected in infected cells: rigid tubular structures assemble in the Golgi region early in infection and frequently connect with mitochondria. In Vero cells, the virus induces an early and spectacular fragmentation of intracellular membranes while productive infection progresses. Assembly occurs in fragmented Golgi stacks and generates tubular structures, as well as the three spherical viral forms. These results, together with our previous studies with nonrelated viruses, show that the Golgi complex contains key factors for the structural transformation of a number of enveloped viruses that assemble intracellularly.

* Corresponding author. Mailing address: Centro Nacional de Biotecnología, CSIC, Campus Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain. Phone: 34-91-5854550. Fax: 34-91-5854506. E-mail: crisco{at}cnb.uam.es.

Journal of Virology, January 2003, p. 1368-1381, Vol. 77, No. 2
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.2.1368-1381.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»