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Journal of Virology, September 2003, p. 10131-10138, Vol. 77, No. 18
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.18.10131-10138.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Hepatitis C Virus-Like Particle Budding: Role of the Core Protein and Importance of Its Asp111

Emmanuelle Blanchard,1 Christophe Hourioux,1,2 Denys Brand,1 Malika Ait-Goughoulte,1 Alain Moreau,1 Sylvie Trassard,2 Pierre-Yves Sizaret,2 Frederic Dubois,1,3 and Philippe Roingeard1,2*

Laboratoire de Virologie,1 Laboratoire de Biologie Cellulaire, Faculté de Médecine et Centre Hospitalier Universitaire, Tours,2 Institut Inter Régional de la Santé, La Riche, France3

Received 8 May 2003/ Accepted 25 June 2003

In the absence of a hepatitis C virus (HCV) culture system, the use of a Semliki Forest virus replicon expressing genes encoding HCV structural proteins that assemble into HCV-like particles provides an opportunity to study HCV morphogenesis. Using this system, we showed that the HCV core protein constitutes the budding apparatus of the virus and that its targeting to the endoplasmic reticulum by means of the signal sequence of E1 protein is essential for budding. In addition, the aspartic acid at position 111 in the HCV core protein sequence was found to be crucial for virus assembly, demonstrating the usefulness of this system for mapping amino acids critical to HCV morphogenesis.

* Corresponding author. Mailing address: Laboratoire de Biologie Cellulaire, Faculté de Médecine, 2 bis Blvd. Tonnellé, 37032 Tours, France. Phone: 33 2 47 36 60 71. Fax: 33 2 47 36 60 90. E-mail: roingeard{at}med.univ-tours.fr.

Journal of Virology, September 2003, p. 10131-10138, Vol. 77, No. 18
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.18.10131-10138.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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