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Journal of Virology, September 2003, p. 10119-10124, Vol. 77, No. 18
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.18.10119-10124.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Analysis of Early Human Immunodeficiency Virus Type 1 DNA Synthesis by Use of a New Sensitive Assay for Quantifying Integrated Provirus

Audrey Brussel and Pierre Sonigo*

Département des Maladies Infectieuses, Institut Cochin, INSERM U567, CNRS UMR 8104, Université René Descartes, 75014 Paris, France

Received 7 February 2003/ Accepted 19 June 2003

A novel Alu-long terminal repeat (LTR)-based real-time nested-PCR assay was developed to quantify integrated human immunodeficiency virus type 1 (HIV-1) DNA in infected cells with both accuracy and high sensitivity (six proviruses within 50,000 cell equivalents). Parallel assays for total HIV-1 DNA and two-LTR HIV-1 DNA circles allowed the synthesis and fate of the different HIV-1 DNA species to be monitored upon a single round of viral replication.

* Corresponding author. Mailing address: Département des Maladies Infectieuses, Institut Cochin, INSERM U567, CNRS UMR 8104, Université René Descartes, 22 rue Méchain, 75014 Paris, France. Phone: 33 1 40 51 64 13. Fax: 33 1 40 51 64 30. E-mail: sonigo{at}cochin.inserm.fr.

Journal of Virology, September 2003, p. 10119-10124, Vol. 77, No. 18
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.18.10119-10124.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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