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Journal of Virology, August 2003, p. 8577-8583, Vol. 77, No. 15
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.15.8577-8583.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Dissection of Cauliflower Mosaic Virus Transactivator/Viroplasmin Reveals Distinct Essential Functions in Basic Virus Replication

Kappei Kobayashi and Thomas Hohn*

Friedrich Miescher Institute, CH-4002 Basel, Switzerland

Received 3 March 2003/ Accepted 16 May 2003

Cauliflower mosaic virus (CaMV) transactivator/viroplasmin (Tav) is an essential multifunctional viral protein. Dissection of Tav by deletion mutagenesis revealed that the central region is essential for CaMV replication in single cells but that the N- and C-terminal parts are not. Strains with mutations in the central region were defective in the translational transactivator function and could be complemented by coexpressing Gag (capsid protein precursor) and Pol (polyprotein with protease, reverse transcriptase, and RNase H activity) from separate monocistronic plasmids. In contrast, total omission of Tav was only partially complemented by Gag and Pol overexpression from separate plasmids. These results indicate that CaMV basic replication requires both Tav-activated polycistronic translation and some posttranslational function(s) of Tav that is not affected by the deletions in the central region of Tav.

* Corresponding author. Mailing address: Friedrich Miescher Institute, P.O. Box 2543, CH-4002 Basel, Switzerland. Phone: 41-61-697-7266. Fax: 41-61-697-3976. E-mail: hohn{at}fmi.ch.

Journal of Virology, August 2003, p. 8577-8583, Vol. 77, No. 15
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.15.8577-8583.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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