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Journal of Virology, July 2003, p. 7863-7871, Vol. 77, No. 14
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.14.7863-7871.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

The Tailless Icosahedral Membrane Virus PRD1 Localizes the Proteins Involved in Genome Packaging and Injection at a Unique Vertex

Brent Gowen,1 Jaana K. H. Bamford,2 Dennis H. Bamford,2 and Stephen D. Fuller1*

The Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford OX3 7BN, United Kingdom,1 Department of Biosciences and Institute of Biotechnology, 00014 University of Helsinki, Finland2

Received 18 November 2002/ Accepted 24 April 2003

The double-stranded DNA (dsDNA) virus PRD1 carries its genome in a membrane surrounded by an icosahedral protein shell. The shell contains 240 copies of the trimeric P3 protein arranged with a pseudo T = 25 triangulation that is reminiscent of the mammalian adenovirus. DNA packaging and infection are believed to occur through the vertices of the particle. We have used immunolabeling to define the distribution of proteins on the virion surface. Antibodies to protein P3 labeled the entire surface of the virus. Most of the 12 vertices labeled with antibodies directed against proteins P5, P2, and P31. These proteins are known to function in virus binding to the cell surface. Proteins P6, P11, and P20 were found on a single vertex per virion. The P6 and P20 proteins are believed to function in DNA packaging. Protein P11 is a pilot protein that is involved in a complex that mediates the early stages of DNA entry to the host cell. Labeling with antibodies to P5 or P2 did not affect the labeling of P6, the unique vertex protein. Labeling with antibodies to the unique vertex protein P6 interfered with the labeling by antibodies to the unique vertex protein P20. We conclude that PRD1 utilizes 11 of its vertices for initial receptor binding. It utilizes a single, unique vertex for both DNA packing during assembly and DNA delivery during infection.

* Corresponding author. Mailing address: The Division of Structural Biology, Wellcome Trust Centre for Human Genetics, The Henry Wellcome Building for Genomic Medicine, University of Oxford, Roosevelt Dr., Headington, Oxford OX3 7BN, United Kingdom. Phone: 44-0-1865-287546. Fax: 44-0-1865-287547. E-mail: stephen.fuller{at}strubi.ox.ac.uk.

Journal of Virology, July 2003, p. 7863-7871, Vol. 77, No. 14
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.14.7863-7871.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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