This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cheng, Q.
Right arrow Articles by Casey, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cheng, Q.
Right arrow Articles by Casey, J. L.

 Previous Article  |  Next Article 

Journal of Virology, July 2003, p. 7786-7795, Vol. 77, No. 14
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.14.7786-7795.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Differential Inhibition of RNA Editing in Hepatitis Delta Virus Genotype III by the Short and Long Forms of Hepatitis Delta Antigen

Qiufang Cheng, Geetha C. Jayan, and John L. Casey*

Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, D.C. 20007-2197

Received 9 January 2003/ Accepted 17 April 2003

Hepatitis delta virus (HDV) produces two essential forms of the sole viral protein from the same open reading frame by using host RNA editing activity at the amber/W site in the antigenomic RNA. The roles of these two forms, HDAg-S and HDAg-L, are opposed. HDAg-S is required for viral RNA replication, whereas HDAg-L, which is produced as a result of editing, inhibits viral RNA replication and is required for virion packaging. Both the rate and amount of editing are important because excessive editing will inhibit viral RNA replication, whereas insufficient editing will reduce virus secretion. Here we show that for HDV genotype III, which is associated with severe HDV disease, HDAg-L strongly inhibits editing of a nonreplicating genotype III reporter RNA, while HDAg-S inhibits only when expressed at much higher levels. The different inhibitory efficiencies are due to RNA structural elements located ca. 25 bp 3' of the editing site in the double-hairpin RNA structure required for editing at the amber/W site in HDV genotype III RNA. These results are consistent with regulation of amber/W editing in HDV genotype III by a negative-feedback mechanism due to differential interactions between structural elements in the HDV genotype III RNA and the two forms of HDAg.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Georgetown University Medical Center, 3900 Reservoir Rd., N.W., Washington, DC 20007. Phone: (202) 687-1052. Fax: (202) 687-1800. E-mail: caseyj{at}georgetown.edu.

Journal of Virology, July 2003, p. 7786-7795, Vol. 77, No. 14
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.14.7786-7795.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Linnstaedt, S. D., Kasprzak, W. K., Shapiro, B. A., Casey, J. L. (2009). The fraction of RNA that folds into the correct branched secondary structure determines hepatitis delta virus type 3 RNA editing levels. RNA 15: 1177-1187 [Abstract] [Full Text]  
  • Defenbaugh, D. A., Johnson, M., Chen, R., Zheng, Y. Y., Casey, J. L. (2009). Hepatitis Delta Antigen Requires a Minimum Length of the Hepatitis Delta Virus Unbranched Rod RNA Structure for Binding. J. Virol. 83: 4548-4556 [Abstract] [Full Text]  
  • PARANA, R., KAY, A., MOLINET, F., VIANA, S., SILVA, L. K., SALCEDO, J. M., TAVARES-NETO, J., LOBATO, C., RIOS-LEITE, M., MATTEONI, L., OLIVEIRA, A. D JR, TAUIL, P., TREPO, C. (2006). HDV GENOTYPES IN THE WESTERN BRAZILIAN AMAZON REGION: A PRELIMINARY REPORT.. Am J Trop Med Hyg 75: 475-479 [Abstract] [Full Text]  
  • Linnstaedt, S. D., Kasprzak, W. K., Shapiro, B. A., Casey, J. L. (2006). The role of a metastable RNA secondary structure in hepatitis delta virus genotype III RNA editing. RNA 12: 1521-1533 [Abstract] [Full Text]  
  • VIANA, S., PARANA, R., MOREIRA, R. C., COMPRI, A. P., MACEDO, V. (2005). HIGH PREVALENCE OF HEPATITIS B VIRUS AND HEPATITIS D VIRUS IN THE WESTERN BRAZILIAN AMAZON. Am J Trop Med Hyg 73: 808-814 [Abstract] [Full Text]  
  • Sato, S., Cornillez-Ty, C., Lazinski, D. W. (2004). By Inhibiting Replication, the Large Hepatitis Delta Antigen Can Indirectly Regulate Amber/W Editing and Its Own Expression. J. Virol. 78: 8120-8134 [Abstract] [Full Text]  
  • Shih, K.-N., Chuang, Y.-T., Liu, H., Lo, S. J. (2004). Hepatitis D virus RNA editing is inhibited by a GFP fusion protein containing a C-terminally deleted delta antigen. J. Gen. Virol. 85: 947-957 [Abstract] [Full Text]  
  • Chang, J., Provost, P., Taylor, J. M. (2003). Resistance of Human Hepatitis Delta Virus RNAs to Dicer Activity. J. Virol. 77: 11910-11917 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»