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Journal of Virology, June 2003, p. 6620-6636, Vol. 77, No. 12
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.12.6620-6636.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Complete Sequence and Genomic Analysis of Rhesus Cytomegalovirus

Scott G. Hansen,1 Lisa I. Strelow,1,2 David C. Franchi,3 David G. Anders,3* and Scott W. Wong1,2,4*

Vaccine and Gene Therapy Institute, Oregon Health & Science University,1 Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, Oregon 97006,2 The David Axelrod Institute, Wadsworth Center, Albany, New York 12201,3 Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, Oregon 972394

Received 21 November 2002/ Accepted 19 March 2003

The complete DNA sequence of rhesus cytomegalovirus (RhCMV) strain 68-1 was determined with the whole-genome shotgun approach on virion DNA. The RhCMV genome is 221,459 bp in length and possesses a 49% G+C base composition. The genome contains 230 potential open reading frames (ORFs) of 100 or more codons that are arranged colinearly with counterparts of previously sequenced betaherpesviruses such as human cytomegalovirus (HCMV). Of the 230 RhCMV ORFs, 138 (60%) are homologous to known HCMV proteins. The conserved ORFs include the structural, replicative, and transcriptional regulatory proteins, immune evasion elements, G protein-coupled receptors, and immunoglobulin homologues. Interestingly, the RhCMV genome also contains sequences with homology to cyclooxygenase-2, an enzyme associated with inflammatory processes. Closer examination identified a series of candidate exons with the capacity to encode a full-length cyclooxygenase-2 protein. Counterparts of cyclooxygenase-2 have not been found in other sequenced herpesviruses. The availability of the complete RhCMV sequence along with the ability to grow RhCMV in vitro will facilitate the construction of recombinant viral strains for identifying viral determinants of CMV pathogenicity in the experimentally infected rhesus macaque and to the development of CMV as a vaccine vector.

* Corresponding author. Mailing address for David G. Anders: The David Axelrod Institute, Wadsworth Center for Molecular Genetics, Albany, NY 12201-2002. Phone: (518) 474-8969. Fax: (518) 474-3181. E-mail: anders{at}wadsworth.org. Scott W. Wong, Vaccine and Gene Therapy Institute, OHSU, West Campus, 505 NW 185th Avenue, Beaverton, OR 97006. Phone: (503) 690-5285. Fax: (503) 418-2719. E-mail: wongs{at}ohsu.edu.

Journal of Virology, June 2003, p. 6620-6636, Vol. 77, No. 12
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.12.6620-6636.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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