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Journal of Virology, June 2003, p. 6551-6555, Vol. 77, No. 11
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.11.6551-6555.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Cell-Free Synthesis of Encephalomyocarditis Virus

Yuri V. Svitkin* and Nahum Sonenberg

Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, Quebec, Canada H3G 1Y6

Received 22 October 2002/ Accepted 10 March 2003

We developed a system for complete replication of encephalomyocarditis virus (EMCV) in a test tube by using an in vitro translation extract from Krebs-2 cells. Efficient virus synthesis occurred in a narrow range of Mg2+ and EMCV RNA concentrations. Excess input RNA impaired RNA replication and virus production but not translation. This suggests the existence of a negative-feedback mechanism for regulation of RNA replication by the viral plus-strand RNA or proteins.

* Corresponding author. Mailing address: Department of Biochemistry, McGill University, 3655 Promenade Sir William Osler, Montreal, Quebec, Canada H3G 1Y6. Phone: (514) 398-5695. Fax: (514) 398-1287. E-mail: yuri.svitkine{at}mcgill.ca.

Journal of Virology, June 2003, p. 6551-6555, Vol. 77, No. 11
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.11.6551-6555.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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