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Journal of Virology, January 2003, p. 776-781, Vol. 77, No. 1
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.1.776-781.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evidence for Packaging of rep-cap Sequences into Adeno-Associated Virus (AAV) Type 2 Capsids in the Absence of Inverted Terminal Repeats: a Model for Generation of rep-Positive AAV Particles

Pascale Nony,{dagger} Gilliane Chadeuf, Jacques Tessier,{ddagger} Philippe Moullier, and Anna Salvetti*

INSERM ERM 0105, CHU Hotel-Dieu, 44035 Nantes, France

Received 3 July 2002/ Accepted 20 September 2002

We previously reported that a 350-bp region of the adeno-associated virus (AAV) type 2 rep gene contains a cis-acting element responsible for the Rep-dependent replication of a transiently transfected rep-cap plasmid. In this study, we further report that replicated rep-cap sequences can be packaged into AAV capsids in the absence of the inverted terminal repeats.

* Corresponding author. Mailing address: Laboratoire de Thérapie Génique, CHU Hotel Dieu, 30 Avenue Jean Monnet, 44035 Nantes Cedex 1, France. Phone: 33 240087490. Fax: 33 240087491. E-mail: salvetti{at}sante.univ-nantes.fr.

{dagger} Present address: CNRS-UMR5641, Domaine Rockefeller, 69373 Lyon Cedex 09, France.

{ddagger} Present address: Department of Molecular Genetics and Microbiology and Gene Therapy Center, College of Medicine, University of Florida, Gainesville, FL 32610.

Journal of Virology, January 2003, p. 776-781, Vol. 77, No. 1
0022-538X/03/$08.00+0     DOI: 10.1128/JVI.77.1.776-781.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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