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Journal of Virology, May 2002, p. 4401-4411, Vol. 76, No. 9
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.9.4401-4411.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Role of Recycling Endosomes and Lysosomes in Dynein-Dependent Entry of Canine Parvovirus

Sanna Suikkanen, Katja Sääjärvi, Jonna Hirsimäki, Outi Välilehto, Hilkka Reunanen, Maija Vihinen-Ranta, and Matti Vuento*

Department of Biological and Environmental Science, University of Jyväskylä, SF-40500 Jyväskylä, Finland

Received 3 October 2001/ Accepted 29 January 2002

Canine parvovirus (CPV) is a nonenveloped virus with a 5-kb single-stranded DNA genome. Lysosomotropic agents and low temperature are known to prevent CPV infection, indicating that the virus enters its host cells by endocytosis and requires an acidic intracellular compartment for penetration into the cytoplasm. After escape from the endocytotic vesicles, CPV is transported to the nucleus for replication. In the present study the intracellular entry pathway of the canine parvovirus in NLFK (Nordisk Laboratory feline kidney) cells was studied. After clustering in clathrin-coated pits and being taken up in coated vesicles, CPV colocalized with coendocytosed transferrin in endosomes resembling recycling endosomes. Later, CPV was found to enter, via late endosomes, a perinuclear vesicular compartment, where it colocalized with lysosomal markers. There was no indication of CPV entry into the trans-Golgi or the endoplasmic reticulum. Similar results were obtained both with full and with empty capsids. The data thus suggest that CPV or its DNA was released from the lysosomal compartment to the cytoplasm to be then transported to the nucleus. Electron microscopy analysis revealed endosomal vesicles containing CPV to be associated with microtubules. In the presence of nocodazole, a microtubule-disrupting drug, CPV entry was blocked and the virus was found in peripheral vesicles. Thus, some step(s) of the entry process were dependent on microtubules. Microinjection of antibodies to dynein caused CPV to remain in pericellular vesicles. This suggests an important role for the motor protein dynein in transporting vesicles containing CPV along the microtubule network.

* Corresponding author. Mailing address: Department of Biological and Environmental Science, University of Jyväskylä, Survontie 9, SF-40500 Jyväskylä, Finland. Phone: 358-14-260-2282. Fax: 358-14-260-2271. E-mail: vuento{at}dodo.jyu.fi.

Journal of Virology, May 2002, p. 4401-4411, Vol. 76, No. 9
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.9.4401-4411.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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