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Journal of Virology, April 2002, p. 4080-4086, Vol. 76, No. 8
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.8.4080-4086.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Preferential Localization of the Epstein-Barr Virus (EBV) Oncoprotein LMP-1 to Nuclei in Human T Cells: Implications for Its Role in the Development of EBV Genome-Positive T-Cell Lymphomas

Jingwu Xu, Ali Ahmad,,{dagger} and José Menezes*

Laboratory of Immunovirology, Department of Microbiology and Immunology, University of Montreal and Ste-Justine Hospital, Montreal, Quebec, Canada H3T 1C5

Received 27 August 2001/ Accepted 16 January 2002

The Epstein-Barr virus (EBV)-encoded latent membrane protein-1 (LMP-1) is thought to play a role in the EBV-induced B-cell transformation and immortalization. EBV has also been implicated in certain human T-cell lymphomas; however, the phenotypic effects of the expression of this oncoprotein in T cells are not known. To learn whether LMP-1 also induces phenotypic changes in T cells, we stably expressed it in human cell lines of T and B lineages and 25 LMP-1-expressing T-cell clones and 7 B-cell clones were examined. Our results show for the first time that, in sharp contrast to B cells, LMP-1 preferentially localizes to nuclei in T cells and does not induce the phenotypic changes in these cells that it induces in B cells, does not associate with TRAF proteins, and does not arrest the cell cycle in the G2/M phase. A computer-assisted analysis revealed that LMP-1 lacks the canonical nuclear localization signal. Our results suggest that this oncoprotein may not play the same role in the lymphomagenesis of T cells as it does in B cells.

* Corresponding author. Mailing address: Laboratory of Immunovirology, Department of Microbiology and Immunology, Ste-Justine Hospital Research Center, 3175 Côte Ste-Catherine, Montreal, Quebec, Canada H3T 1C5. Phone: (514) 345-4691. Fax: (514) 345-4801. E-mail for J. Menezes: jmenezes{at}justine.umontreal.ca.

{dagger} E-mail for A. Ahmad: ahmada{at}justine.umontreal.ca.

Journal of Virology, April 2002, p. 4080-4086, Vol. 76, No. 8
0022-538X/02/$04.00+0     DOI: 10.1128/JVI.76.8.4080-4086.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.





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